病原学检查方法课件

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,Parasitic Pathogen Examination,寄生虫病原检查,Parasitic Pathogen,1,Etioligic Diagnosis,Stool,blood,marrow,urine,sputum,humores(,体液,),secretion and tissue.,Purpose of examination,Examining pathogen,identifying the species,of parasite and the degree of infection.,Specimen:,Etioligic DiagnosisStool,blo,2,Blood and marrow examination,Examining plasmodium,the collection of blood,time：,blood smear,thin smear：,thick smear：,stain,Wright stain,Giemsa stain,Blood and marrow examination,3,Practice 1,Practicing thin smear,stain and,observing,Plasmodium berghei,.,Practice 1 Practicing thin,4,薄血膜制片,厚血制片,取血与涂片,薄血膜制片 取血与涂片,5,固定与染色,固定,薄血膜晾干后，甲醇固定,染色,将姬氏染液滴于已固定的薄血膜上，染色半小时（室温），自来水冲洗。血片晾干后镜检。,固定与染色 固定,6,Blood and marrow examination,Examining microfilaria from blood,Time for blood collecting,Methods:,Examining,L.donovani,from marrow,Aspiration,(,穿刺检查,):,Blood and marrow examination,7,Stool Examination,Collect the stool in a dry,clean,leakproof container.Make sure no urine,water,soil or other material gets in the container.,Label the container with the patients name,date and time of collection.,Significance:,Caution:,Stool Examination Collect the,8,Stool Examination,Before the patient taking medicine the stool specimen should be collected.,Fresh stool should be examined,or preserved immediately.,Stool Examination Before the,9,Macrography,(,肉眼观察）,Wet mount examination,(,Direct smear,),Concentration methods,Larva hatching methods,How to find parasites in the feces?,Macrography(肉眼观察）How to fi,10,Stool Examination,Wet mount method:,Direct smear with saline,Iodine staining method,（碘染色法）,Stool Examination Wet mount m,11,Stool Examination,Concentration method:,modified Kato-Katzs method,sedimentation methods,flotation methods,Stool Examination Concentrati,12,Flotation method:,Stool Examination,Zinc sulfate centrifugal flotation,Saturated brine flotation,Sucrose sugar flotation,Flotation method:Stool Exami,13,gravitational,sedimentation method,自然沉淀法,centrifugal,sedimentation method,离心沉淀法,Stool Examination,Sedimentation method:,larva hatching method:,miracidium hatching method,culture method for hookworm larva,gravitational sedimentation m,14,How to confirm the density of,parasite or worm burden?,Modified Kato-Katzs method,Stolls eggs counting,How to confirm the density,15,Practice 2,Self-examining stool yourself!,Direct smear with saline,Saturated brine flotation,Practice 2Self-e,16,病原学检查方法课件,17,Examining parasite from perianal skin,Parasite,eggs and adults of pinworm,eggs of,Taenia,Methods,cellophane tape method（透明胶纸法）,cotton swab method（棉拭子法）,Examining parasite from peria,18,Sputum,Urine,Vaginal secretion,Duodenal fluid,Humores,CSF-,Sputum,Urine,Vaginal,19,Biopsy and animal inoculation,Biopsy and animal inoculation,20,Summary,Principles of different methods of parasitic diagnosis.(you should master them.),The processes of experiments.,Summary Principles of differen,21,Preparing for the next class,Contents:,Principles,Experiments,Medical terms,Preparing for the next class C,22,