资源描述
单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,DNA,倍体和细胞周期分析,细胞周期和,DNA,倍体分析的原理,G0/G1,期,DNA,含量为,2C,S,期细胞,DNA,含量开始增加,2C,G2/M,期细胞,DNA,含量开始增加至,4C,1,、正常细胞,DNA,含量稳定在,2C,水平,2,、性细胞,DNA,含量为,1C,3,、处于增殖期至分裂期细胞由,2C,增加至,4C,4,、凋亡细胞,DNA,断裂,含量,1.1,或,2.1,或,15%,的,S,期细胞，并伴有,G0/G1,峰的,CV9%,，提示为肿瘤或增生旺盛的病变,；,如无明显异倍体，但,G0/G1,峰的,CV,增大，并伴有,10%15%,的,S,期细胞和一个突出的,4,倍体峰，诊断为可疑肿瘤或增生旺盛的病变。,发现癌前病变，协助肿瘤早期诊断,在肿瘤的诊断、预后判断和治疗中的作用,在指导肿瘤化疗中的作用,【,临床意义,】,乳腺癌,DNA,异倍体患者生存率,乳腺癌肿瘤细胞异倍体患者长期生存率显著下降,【,临床病例,】,Diploid:46.75%,Dip G1:97.54%at 46.06,Dip G2:0.58%at 92.12,Dip S:1.88%G2/G1:2.00,%CV:2.26,Aneuploid 1:47.55%,An1 G1:93.05%at 49.83,An1 G2:0.00%at 99.67,An1 S:6.95%G2/G1:2.00,%CV:2.13 DI:1.08,Aneuploid 2:5.70%,An2 G1:94.97%at 55.72,An2 G2:5.03%at 103.44,An2 S:0.00%G2/G1:1.86,%CV:4.52 DI:1.21,AL,骨髓,Diploid:64.50%,Dip G1:92.45%at 49.24,Dip G2:2.30%at 98.48,Dip S:5.26%G2/G1:2.00,%CV:3.21,Aneuploid 1:35.50%,An1 G1:97.69%at 58.53,An1 G2:0.00%at 123.89,An1 S:2.31%G2/G1:2.12,%CV:3.14 DI:1.19,Total Aneuploid S-Phase:2.31%,Total S-Phase:4.21%,Total B.A.D.:0.35%,Debris:1.14%,Aggregates:0.00%,Modeled events:3567,All cycle events:3526,Cycle events per channel:47,RCS:2.356,MM,骨髓,宫颈脱落细胞,Diploid:97.61%,Dip G1:97.74%at 49.78,Dip G2:0.02%at 99.57,Dip S:2.25%G2/G1:2.00,%CV:6.49,Aneuploid 1:2.39%,An1 G1:84.90%at 75.79,An1 G2:15.10%at 109.99,An1 S:0.00%G2/G1:1.45,%CV:3.66 DI:1.52,肺癌 胸水,Diploid:100.00%,Dip G1:55.08%at 46.83,Dip G2:15.67%at 92.69,Dip S:29.25%,G2/G1:1.98,%CV:7.02,增殖旺盛的肿瘤细胞株,
展开阅读全文