Amnis-纳米课件

,Thursday, August 29, 2024,纳米颗粒靶向进入目的细胞的量化分析,纳米粒药物载体,调节释药速度,改变药物在体内的分布,纳米乳药物载体,提高难溶性药物溶解度,提高药物生物利用度,磁性纳米载体,减少药物毒副作用,准确到达特定的靶向部位,纳米药物的优势,Mid Internalization,Low Internalization,High Internalization,纳米颗粒是否进入细胞,内化参数, Internalization,细胞内荧光值与细胞内外荧光值的比值,Brightfield,Babesia YOYO1,Single Parasite,Two Parasites,Three Parasites,纳米颗粒进入细胞的程度,计点参数,spot count,图像中点的数量,分析纳米颗粒靶向进入目的细胞的原理,Baginskiy I, Lai TC, and Tsai DP, et al. Chitosan-Modified Stable Colloidal Gold Nanostars for the Photothermolysis of Cancer Cells. J. Phys. Chem. C 2021, 117, 23962410.,Au NRs,进入,J5,肝癌细胞的效率高于,Au NSs,Blank,10 ug/ml chitosan capped Au NSs,4 ug/ml chitosan capped Au NSs,10 ug/ml chitosan capped Au NRs,4 ug/ml chitosan capped Au NRs,FITC-Au,DAPI,FITC-Au,DAPI,FITC-Au,DAPI,FITC-Au,DAPI,FITC-Au,DAPI,NRs: nano rods,NSs: nano star shape,碳纳米管,/,石墨烯,的研究难点,容易受到细胞内碳聚集物的干扰,由于探针容易发生解离，所以不能像其他纳米材料那样利用探针研究其在细胞中的代谢过程,Marangon I, Gazeau F, et al.,Intercellular carbon nanotube translocation assessed by flow cytometry imaging.,Nano Lett. 2021 Sep 12;12(9):4830-7. (IF 13.198),通过能量依赖内化途径，,碳纳米管,/,石墨烯,进入的细胞,碳纳米管,/,石墨烯,进入内皮细胞的效率,Johnston AP, Caruso F, et al.,Targeting cancer cells: controlling the binding and internalization of antibody-functionalized capsules.,ACS Nano. 2021 Aug 28;6(8):6667-74.,(IF 10.774),huA33 mAbAz functionalized PVPONAlk capsules,LIM2405+CRC cells,Surface bound capsules.,(b) Cells with 1-2 internalized capsules.,(c) Cells with multiple internalized capsules.,抗体标记的多聚纳米载体药物特异性打靶结肠癌细胞,LIM2405+CRC cells:,表达,HuA33,抗原,LIM2405-CRC cells:,不表达,HuA33,抗原,lin 1 (CD3, CD14, CD16, CD19, CD20, and CD56),LbL,微囊包裹多肽疫苗可以有效的进入短尾猿外周血细胞、激活,T,细胞免疫,De Rose, R., A. N. Zelikin, et al.,Binding, Internalization, and Antigen Presentation of Vaccine-Loaded Nanoengineered Capsules in Blood.,Advanced Materials, 2021, 20(24): 4698-4703. (IF 13.877),Rhodamine B,Lin 1-/HLA-DR+,Lin 1+/HLA-DR+,Lin 1+/HLA-DR-,使用Amnis ImageStream发表的文章,Johnston AP, Caruso F, et al.,Targeting cancer cells: controlling the binding and internalization of antibody-functionalized capsules.,ACS Nano. 2021 Aug 28;6(8):6667-74. (IF 10.774),Yan Y, Caruso F, et al.,Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for Doxorubicin delivery to colorectal cancer cells.,ACS Nano. 2021 May 25;4(5):2928-36. (IF 10.774),De Rose, R., A. N. Zelikin, Caruso F, et al.,Binding, Internalization, and Antigen Presentation of Vaccine-Loaded Nanoengineered Capsules in Blood.,Advanced Materials, 2021, 20(24): 4698-4703. (IF 13.877),金牌客户,Professor Frank Caruso,Department of Chemical and Biomolecular Engineering,The University of Melbourne,Australia,澳大利亚科学院院士,被Thomas Reuter评为近二十年最伟大的20位材料科学家之一,由于其在纳米科学和技术方面的奉献，获得2021年ACS Nano Lectureship Awards,Thanks for your attention !,