细胞工程英文简介培训课件

细细胞工程英文胞工程英文简简介介10/2005细胞工程英文简介CE0-2CellEngineering-Engineering is the applied science of acquiring and applying knowledge to design,analysis,and/or construction of works for practical purposes.10/2005细胞工程英文简介CE0-3Cell EngineeringWhatWhenWhoWhereHow10/2005细胞工程英文简介CE0-410/2005细胞工程英文简介CE0-5Wheat,Tree,Cotton,Foot,fruit,vegetable,cotton,timber,PlantPig,Cattle,Sheep,Poultry,Meat,eggs,milk,fur,BreedingCellCellBreeding,biological product,medicine,Cultivationand ModificationWhat is Cell Engineering10/2005细胞工程英文简介CE0-6Monoclonalantibodyproductionanexampleofcellengineering10/2005细胞工程英文简介CE0-7CELecture0IntroductiontoCellEngineering10/2005细胞工程英文简介CE0-8CE0-1CellEngineeringandBiotechnologyIntroductiontoBiotechnologyWhatisCellEngineeringHowCellEngineeringRelatedtootherdisciplinesCE0-2DevelopmentofCellEngineeringCE0-3ApplicationAreasofCellEngineeringCE0-4ImportantconceptsinCellEngineeringCE0-5TeachingSchedule10/2005细胞工程英文简介CE0-9CE0-1CellEngineeringandBiotechnology10/2005细胞工程英文简介CE0-10Cell engineeringencompassesawiderangeofresearchandpracticaltechniquesinscience.Technically,anymanipulationthatdisturbsacellfromitsoriginal,naturalstateiscellengineering.ABiotechnologyBWhatisCellEngineeringCCellEngineeringandotherdisciplines10/2005细胞工程英文简介CE0-11IntroductiontoBiotechnologyTraditional BiotechnologyFermentation,GeneticBreeding,Modern BiotechnologyToachieveaboveobjectivesbychangingthepropertiesoforganismsandmoleculesusingmodernbiochemicalandmolecularbiologytechniques10/2005细胞工程英文简介CE0-12Milestone:Recombinant DNA technique in 1970s 1944DNAandgeneticinformation1953DNAmodelandreplicatio1961Geneticcode1972RecombinantDNAtechniquesMilestone:GeneticEngineering,thecoreofBiotechnololgy10/2005细胞工程英文简介CE0-13Biotechnology is technology based onbiology,especiallywhenusedinagriculture,food science,and medicine.The UNConventiononBiologicalDiversityhascomeup with one of many definitions ofbiotechnology:Biotechnology means any technologicalapplication that uses biological systems,living organisms,or derivatives thereof,tomake or modify products or processes forspecificuse.Biotechnology can also be defined as themanipulation of organisms to do practicalthingsandtoprovideusefulproducts.10/2005细胞工程英文简介CE0-14Biological ResourcesOrganisms or parts of organismMolecules10/2005细胞工程英文简介CE0-15Oneaspectofbiotechnologyisthedirecteduseoforganismsforthemanufactureoforganicproducts(examplesincludebeerandmilkproducts.Foranotherexample,naturallypresentbacteriaareutilizedbytheminingindustryinbioleaching.Biotechnologyisalsousedtorecycle,treatwaste,cleanupsitescontaminatedbyindustrialactivities(bioremediation),andproducebiologicalweapons.Therearealsoapplicationsofbiotechnologythatdonotuselivingorganisms.ExamplesareDNAmicroarraysusedingeneticsandradioactivetracersusedinmedicine.10/2005细胞工程英文简介CE0-1610/2005细胞工程英文简介CE0-17ExamplesGenetic EngineeringCell EngineeringEnzyme EngineeringFermentation EngineeringBiotechnologyHowthesetechniquesrelatedtoeachother10/2005细胞工程英文简介CE0-18DNARNAProteinTranscriptionTranslationRegulationBCTCentral DogmaIntroduction to BiotechnologyReplication10/2005细胞工程英文简介CE0-19Somebasicbiologicaltechniques：CultivationTechniquesCytologyandHistologyTechniquesMolecularBiologyTechniquesBiochemicalTechniquesIntroduction to Biotechnology10/2005细胞工程英文简介CE0-20Cell（Tissue，Organ）品种改良，生物制品品种改良，生物制品(如抗如抗体制备体制备)，医学用途，医学用途，Cultivationand ModificationSkitransgenicmouse(lowerleft)andnon-transgeniclittermate(upperright)(SutraveandHughes)thisshowedthatSkimodulatedskeletalmuscledevelopmentandmaturationWhatisCellEngineering10/2005细胞工程英文简介CE0-21细胞工程学的核心对细胞的培养和改造Cultivation and ModificationCell（Tissue，Organ）品种改良，生物制品品种改良，生物制品(如抗如抗体制备体制备)，医学用途，医学用途，Cultivationand Modification10/2005细胞工程英文简介CE0-22Genetic Operation LevelDNA,chromosome,cell,Application areasAnimal，plant，medicine，basicresearchPurposeBioproducts,Cell（Tissue，Organ）品种改良，生物制品品种改良，生物制品(如抗如抗体制备体制备)，医学用途，医学用途，Cultivationand Modification10/2005细胞工程英文简介CE0-23Features of Cell Engineering-承上启下的学科承上启下的学科-交叉学科交叉学科 Multi-discipline各学科在理论和技术各学科在理论和技术(Theories and Techniques)上上的承上启下和交叉：的承上启下和交叉：生命科学自身各学科的交叉生命科学自身各学科的交叉 Intra-discipline生命科学同其他学科的交叉生命科学同其他学科的交叉 Inter-disciplineHow Cell Engineering related to other disciplines10/2005细胞工程英文简介CE0-24Cell BiologyDevelopment BiologyGeneticsBiophysicsMolecular BiologyPhysiologyBiochemistryCell Engineering蛋白质工程蛋白质工程BiologicalProduct酶工程酶工程Biomedicine动植物改良动植物改良Genetic EngineeringHow Cell Engineering related to other disciplines10/2005细胞工程英文简介CE0-25Cell BiologyBiochemistryPhysiologyMolecular BiologyGeneticsBiophysicsDevelopment BiologyHow Cell Engineering related to other disciplines10/2005细胞工程英文简介CE0-26CE0-2DevelopmentofCellEngineering10/2005细胞工程英文简介CE0-27“Eachaddstoourknowledgeofnatureandfromallthefactsassembledarisesgrandeur.”Aristotle(384-322 B.C.)10/2005细胞工程英文简介CE0-28WHYHISTORY?Thosewhocannotrememberhistorywillbecondemnedforrepeat!10/2005细胞工程英文简介CE0-29WHYHISTORY?Placesmoderndevelopmentsincontext-ie.Notisolateddiscoveriesbutbuiltuponyearsofinquiry.Demonstratesconnectednessofdiversefieldsofscience-forexamplestudiesinnutritionledtotissuecultureadvances;tissuecultureexperimentscontributedtothediscoveryofplanthormonesputspersonalitiesintotheprocess10/2005细胞工程英文简介CE0-30How scientific theory developsHypothesisTest experimentorobservationsSupport hypothesis(maybetrue)Hypothesis becomes theory理论指导下的改造自然理论指导下的改造自然Reject hypothesisModify AbandonObservation10/2005细胞工程英文简介CE0-31Cell Theory-proposed in 1830sSchleiden,Matthias(1804-1881)GermanbotanistandmastermicroscopistSchwann,Theodor(1810-1882)Germanphysiologist10/2005细胞工程英文简介CE0-33MT Burrows and A CarrelTumors and cancer cells were grown in flasks for the first time early in the 20th century.Pioneers Alexis Carrel and Montrose Burrows developed the first long-term tissue cultures in 1911.Burrows coined the phrase tissue culture to describe their procedure.A Carrel(1873 1944)The Nobel Prize in Physiology or Medicine 1912 in recognition of his work on vascular suture and the transplantation of blood vessels and organs10/2005细胞工程英文简介CE0-34IsolationandFusionofProtoplastCocking10/2005细胞工程英文简介CE0-35FusionofanimalcellsandmonoclonalantibodyGeorges J.F.KhlerCsar Milstein 1984 Nobel Prize Winners10/2005细胞工程英文简介CE0-36TransgenicAnimalsTransgenicmiceThefirstsuccessfulproductionoftransgenicmiceusingpronuclearmicroinjectionwasreportedin1980(Gordonetal.,1980)Transgenicfish198410/2005细胞工程英文简介CE0-37Dolly 10/2005细胞工程英文简介CE0-38CE0-3ApplicationAreasofCellEngineering10/2005细胞工程英文简介CE0-39Plant Cell EngineeringAnimal Cell EngineeringBiomedicineBasic Theory10/2005细胞工程英文简介CE0-40CE0-4ImportantconceptsinCellEngineeringC CellCultureandTissueCultureellCultureandTissueCulture10/2005细胞工程英文简介CE0-41细胞培养(cellculture):细胞的离体培养组织培养(tissueculture):组织的离体培养InvitroandinvivoCell（Tissue，Organ）品种改良，生物制品品种改良，生物制品(如抗如抗体制备体制备)，医学用途，医学用途，Cultivationand Modification10/2005细胞工程英文简介CE0-42CE0-5TeachingSchedule10/2005细胞工程英文简介CE0-43细胞工程细胞工程 Cell Engineering细胞工程实验室基本实验技术BasicLaboratoryTechniques细胞的全能性与形态发生Totipotencyandmorphogenesis植物细胞工程Plantcellengineering植物细胞培养及组织培养植物繁殖的新途径(微型繁殖，作物消毒，人工种子)植物细胞培养与次生产物生产植物原生质体培养及细胞杂交技术作物新品种的培育1(突变体的利用，单倍体育种)作物新品种的培育2(植物基因工程)动物细胞工程Animalcellengineering动物细胞培养动物细胞融合及单克隆抗体干细胞工程Stemcellengineering转基因动物Transgenicanimal动物体细胞核移植技术及克隆动物组织工程组织工程Tissue Engineering概念应用10/2005细胞工程英文简介CE0-44掌握掌握(Master)(Master)细胞工程相关的重要理论重要理论理解理解(Understand)(Understand)细胞工程的各种技术的基本原理及应用基本原理及应用了解了解(Know)(Know)细胞工程领域的最新进展进展和发展前景前景培养培养(Have)(Have)针对具体细胞工程课题进行设计和评价的能力设计和评价的能力设计和评价的能力设计和评价的能力(Design and(Design and(Design and(Design and EveluationEveluationEveluationEveluation)10/2005细胞工程英文简介CE0-45关于教材Cell and Molcecular Biology影印版GeraldKarp主编,高等教育出版社，2006年。AnIntroductiontoMetabolicandCellularEngineering影印版SCortassa等,科学出版社，2004年。IntroductiontoCellandTissueCulture:TheoryandTechniqueJennieP.MatherandPenelopeE.Roberts,PlenumPress,NewYork,1998.细胞工程安利国主编2005年科学出版社10/2005细胞工程英文简介CE0-46关于考核From 16-Feb to 31-Mar Study 105 projects inhttp:/www.grandchallenges.org/Explorations/Pages/GrantsAwarded.aspx Give introduction for at least one project.From 31-Mar to 31-May Write one proposal.10/2005细胞工程英文简介CE0-47TheEnd