简介WatersUPLC故障排除专题培训ppt课件

2013 Waters Corporation 1Waters UPLC Troubleshooting Training Waters UPLC Troubleshooting Tr2013 Waters Corporation 2AgendaAgendaACQUITY UPLC Family：UPLC&H-ClassWaters UPLC SystemUPLC Solvent Management (BSM)UPLC Sample Management (SM)Waters ACQUITY H-Class SystemQuaternary Solvent Manager(QSM)Sample Manager-Flow Through Needle(SM-FTN)Waters UPLC Detector Troubleshooting TrainingWaters UPLC PDA and PDAeTUVFLRELSDOpen DiscussionAgendaACQUITY UPLC Family：UPL2013 Waters Corporation 3ACQUITY UPLC FamilyACQUITY UPLC FamilyACQUITY UPLC Family2013 Waters Corporation 4ACQUITY UPLC SystemACQUITY UPLC SystemACQUITY UPLC System2013 Waters Corporation 5ACQUITY UPLC SystemACQUITY UPLC SystemDetectors:Optical and/or Mass SpecTunable UV or Photodiode ArrayOptimized flow cell for UPLCHigh speed data samplingColumn Manager:Innovative pivot designPositions column to detectorE-Cord connectionSample Manager:Low dispersion XYZZ FormatFast cycle timesLow carryoverPlates and/or vialsOptional Sample OrganizerBinary Solvent Manager:High pressure blendingBinary gradientsFour solvent choicesOn-line degassingLow dispersion designUPLC pressure capabilitiesSample Organizer:(option)Expands capacity(22/15/8)Shuttles plate feedHeated/chilledSystem Considerations:Small FootprintRedesigned tubing and fittingsConsolidated waste managementIntegrated system diagnosticsConnections Insight remote diagnosticsACQUITY UPLC SystemDetectors:C2013 Waters Corporation 6A1 A1 A2A2B1B1B2B2WasteFluidics Fluidics and Connectionsand ConnectionsTubing management Chromatographic solvents:oA1/A2,B1/B2Two Sample Manager wash solventsSeal WashCommon fittings and tools for usersColumn inlets/outlets and fittings oFinger tight,turn(use 1/4”wrench)Dedicated tubing3 piece compression screw and ferruleSmall footprint 13”wide24.25”with Sample OrganizerA1 A2B1B2WasteFluidics and Co2013 Waters Corporation 7UPLCSolvent ManagerUPLCSolvent Manager2013 Waters Corporation 8ACQUITY UPLC System ACQUITY UPLC System Solvent DeliverySolvent Delivery 15 K psi performanceAccurate and precise flow ratesAccurate and precise compositionLow delay volume for gradient applicationso5%Ethyl AcetateToluene Chlorinated solvents:(Trichlorobenzene)Recommended Solvents ListACQUI2013 Waters Corporation 14UPLCSample ManagerUPLCSample Manager2013 Waters Corporation 15Sample Manager Fluidics TraySample Manager Fluidics Tray Pressure Transducer Valve Manifold Drive Gears Wash SyringesWeak Wash Strong WashMetering Syringe Drive Assembly Sample Manager Fluidics Tray 2013 Waters Corporation 16Metering Syringe Valve3 Way Rotary ValveMetering Syringe AssemblyMetering Syringe AssemblySample Syringe50 uL volume PN 410001348100 L volume PN 700002570250 L volume PN 410001347ofor use with 50 L loopMetering Syringe ValveMetering2013 Waters Corporation 17Column Heating CompartmentColumn Heating CompartmentColumn Heating Compartment2013 Waters Corporation 18ACQUITY In-Line FilterACQUITY In-Line Filter18ACQUITY In-Line Filter182013 Waters Corporation 19Column In-Line Filter AssemblyColumn In-Line Filter AssemblyColumn In-Line Filter Assembly2013 Waters Corporation 20Column In-Line Filter AssemblyColumn In-Line Filter AssemblyColumn In-Line Filter Assembly2013 Waters Corporation 21Column HeatingColumn HeatingColumn Heating2013 Waters Corporation 22Sample Manager Flow PathSample Manager Flow PathSample Manager Flow Path2013 Waters Corporation 23Available Loops Available Loops Description P/NStatus1 L Loop430001516Optional2 L Loop430001264Optional5 L Loop430001233Optional10 L Loop430001326Standard20 L Loop430001320Optional 50 L Loop430001325Optional100 L Loop170001792(Kit)Optional250 L Loop176001780(Kit)OptionalAvailable Loops DescriptionP/2013 Waters Corporation 24Volume Configuration MenuVolume Configuration MenuBefore running the Characterize Needle and Loop Volume function,make sure the proper volumes are configured in the Volumes Configuration menu.The above values are standard for currently shipping ACQUITY system.Volume Configuration MenuBefor2013 Waters Corporation 25Recommended Injection Volumes,Recommended Injection Volumes,Loop Sizes and Injection ModesLoop Sizes and Injection ModesLoop Volume(uL)Partial Loop w/Needle Overfill Mode Recommended sample injection volume range(uL)Partial Loop(Pressure Assist Mode)Recommended sample injection volume range(uL)Full Loop Mode Recommended sample injection volume(uL0Automatic overfill factor1uL0.2 to 0.75Not Recommended1.05.82uL0.4 to 1.5Not Recommended2.05.65uL1.0 to 3.8Not Recommended5.0510uL2.0 to 7.52.0 to 5.010.0420uL4.0 to 15.04.0 to 10.020.0350uL10.0 to 37.510.0 to 25.050.02100uL20.0 to 75.020.0 to 50.0100.02250uL40.0 to 150.040.0 to 125.0250.01.5Recommended PLNO injection volume 20%to 75%of loop volumeRecommended Partial Loop Injection 20%to 50%of loop volumeRecommended Injection Volumes,2013 Waters Corporation 26ACQUITY H-Class SystemACQUITY H-Class SystemQSMQuaternary Solvent Manager SM-FTNSample Manager-Flow Through Needle CH-AHigh Temperature Column HeaterActive Pre-Heating DetectorsTUV,PDA ePDA,FLR,ELSDACQUITY H-Class SystemQSMSM-FT2013 Waters Corporation 27The ACQUITY H-Class System consists of the following:Quaternary Solvent Manager(QSM)Four solvent low pressure gradient mixing Supports UPLC PressuresLow gradient delay volumeSM-FTN(Sample Manager-Flow Through Needle)One injection mode-direct inject typeMuch simpler-Just specify injection volumeExcellent recovery and precisionCH-AHigh temperature column heater with active pre-heatingACQUITY H-Class SystemACQUITY H-Class SystemThe ACQUITY H-Class System con2013 Waters Corporation 28QSM Fluidics-Front ViewQSM Fluidics-Front ViewPrimary HeadAccumulator HeadVent Valve100ul MixerGradient Proportioning Valve PairsLow PressureManifoldDegasser ChambersSeal Wash PumpQSM Fluidics-Front ViewPrimary2013 Waters Corporation 29QSM Console Top Level MenuQSM Console Top Level MenuQSM Console Top Level Menu2013 Waters Corporation 30ACQUITY H-Class SystemACQUITY H-Class SystemACQUITY H-Class System2013 Waters Corporation 31简介WatersUPLC故障排除专题培训ppt课件2013 Waters Corporation 32SMDI Front Panel FluidicsSMDI Front Panel FluidicsMetering Syringe ValveCheck Valve and UnionInject ValveWash Solvent Inlet Line(not degassed)Leak Sensor ConnectionsSample Metering SyringeSMDI Front Panel FluidicsMeter2013 Waters Corporation 33SDMI Inject Valve ConnectionsSDMI Inject Valve ConnectionsTo Check ValveTo CH-A and ColumnStationary Side TubeFrom PumpTo Pressure TransducerTo Sample NeedleSDMI Inject Valve ConnectionsT2013 Waters Corporation 34Sample CompartmentSample CompartmentPower and Status LEDsPlate Selector SwitchRotary Tray(Plate Holder)Magnetic Door SensorPower SwitchSample CompartmentPower and St2013 Waters Corporation 35SM-FTN Interactive Display MenuSM-FTN Interactive Display MenuSM-FTN Interactive Display Men2013 Waters Corporation 36CH-ACH-AImproved Door GasketAdded Tubing Gasket(top and bottom)For 3rd party Mass Spec at left side ofACQUITY Stack.Allows use of existingPassive Pre-heaterNo wires to connectActive Pre-heaterCH-AImproved Door GasketAdded 2013 Waters Corporation 37Installing the Active Pre-HeaterInstalling the Active Pre-HeaterInstalling the Active Pre-Heat2013 Waters Corporation 38Gold Plated Compression ScrewPEEK FerruleStainless Steel Cap NutStainless Steel Tubing(with brazed on collet)Installing the Active Pre-HeaterInstalling the Active Pre-HeaterGold Plated Compression ScrewP2013 Waters Corporation 39UPLCDetectorsUPLCDetectors2013 Waters Corporation 40DetectorsDetectorsSupports all current ACQUITY detectorsPDA and PDAeTUVFLRELSDDetectorsSupports all current 2013 Waters Corporation 41ACQUITY UPLC PDAACQUITY UPLC PDAACQUITY UPLC PDA2013 Waters Corporation 42ACQUITY UPLC PDA PM kitsACQUITY UPLC PDA PM kitsACQUITY UPLC PDA PM kits2013 Waters Corporation 43ACQUITY UPLC PDA Optics AssemblyACQUITY UPLC PDA Optics Assemblyand Spectrumand SpectrumACQUITY UPLC PDA Optics Assemb2013 Waters Corporation 44ACQUITY UPLC TUVACQUITY UPLC TUVACQUITY UPLC TUV2013 Waters Corporation 45ACQUITYACQUITY UPLCUPLC TUVTUV Optics AssemblyOptics AssemblyACQUITY UPLC TUV Optics Assemb2013 Waters Corporation 46ACQUITYACQUITY UPLCUPLC TUVTUV Optics AssemblyOptics AssemblyACQUITY UPLC TUV Optics Assemb2013 Waters Corporation 47ACQUITY UPLC FLRACQUITY UPLC FLRACQUITY UPLC FLR2013 Waters Corporation 48ACQUITYACQUITY UPLCUPLC FLRFLR Optics AssemblyOptics AssemblyACQUITY UPLC FLR Optics Assemb2013 Waters Corporation 49ACQUITY UPLC ELSDACQUITY UPLC ELSDACQUITY UPLC ELSD2013 Waters Corporation 50Questions?Questions?2013 Waters Corporation 51简介WatersUPLC故障排除专题培训ppt课件