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生物化学与分子生物学PPT素材可根据需要选择取舍生物化学与分子生物学PPT素材可根据需要选择取舍Metabolism of 5-fluorouracil(5-FU)5-FUCarac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridine phosphorylase5-FluorodeoxyuridineThymidine phosphorylaseOrotatephosphoribosyltransferaseThymidine kinase5-FUTP5-FdUTPRNADNA5-nucleotidase5-nucleotidaseUridine kinaseATPADPATPADPPRPPMetabolism of 5-fluorouracil(2Western Blotting1.Resolve protein samples on SDS-PAGE6.Incubate the blot with chemiluminescent HRP substrate and expose to film 2.Electrophoretically transfer fractionated proteins from gel onto PVDF membrane3.Block the membrane with neutral protein (BSA or milk casein)4.Incubate the membrane with primary antibody specific to target protein5.Incubate the membrane with HRP-labeled secondary antibody specific to primary antibodyWestern Blotting1.Resolve pro3分子生物学之PPT课件素材精编4分子生物学之PPT课件素材精编5CholesterolSterol ToolkitPregnenolonProgesteronAldosteronCortisolCorticosteroneAndrostendioneTestosteronEstriol17-Estradiol1,25-DihydroxycholecalciferolCholic acidCholesterolSterol ToolkitPregn6Covalent modification of proteinsPoly(ADP ribosyl)Polymerase(PARP)Poly(ADP ribosyl)polymerase(PARP)NADNicotinamidePoly(ADP ribosyl)polymerase(PARP)Poly(ADP ribosyl)chain elongationand branchingNicotinamideNADNADDNA polymerase DNA polymerase DNA polymerase Covalent modification of prote7OligosacharyltransferaseN-glycosylation pathway in ERMann-P-DolicholtransferasesFlippaseDolicholPDolicholPPDolicholPPDolicholPPDolicholPPGlcNAc-PtransferaseGlcNActransferaseDolicholPDolicholPPDolicholPPGDP-Mannosyl transferasesGlc-P-DolicholtransferasesDolicholPDolichol-pyrophofphataseCytosolER40S60SOligosacharyltransferaseN-gly8UTPCTPTMPPyrimidine Nucleotide InterconversionsUMPATPADPH2OUDPNucleosidemonophosphatekinaseATPADPNucleosidediphosphatekinaseATPADPCTPsynthaseRibonucleotidereductasedeoxyUDPNucleosidediphosphatephosphohydrolasedeoxyUMPThymidylatesynthaseN5,N10-MethyleneTHFDHFTTPNucleosidemonophosphatekinaseNucleosidediphosphatekinaseUTPCTPTMPPyrimidine Nucleotide9Myo-Inositol-3-phosphateIns(3)P1Biosynthesis and metabolism of phosphoinositidesGlucose 6-pgosphateIns(3)P1synthaseIns(3)P1phosphataseMyo-InositolInsPtdInssynthasePtdInsMyo-inositolkinasePtdIns-4-PPtdIns4-kinasePtdIns5-kinasePtdIns-4,5-P2PI3KPtdIns-3,4,5-P3PLCPLCPI3KPtdIns-3,4-P2Ins-1,3,4-P3Ins-1,3,4,5-P4Ins-1,4,5-P3PLCPTENPTENIns-1,4-P3PTENPTENMyo-Inositol-3-phosphateIns(310Fatty acidsynthase-ketoacylACP-synthaseFatty acids synthesisAcetyl CoAcarboxylaseATPADP+PiAcetyl CoAMalonyl CoACitrateOxaloacetateATPADP+PiCitrate lyaseAcetyltransacylaseMalonyltransacylasePantetheine-ketoacylACP-reductasePantetheinePantetheine-hydroxyacylACP-dehydratasePantetheineEnoyl-ACPreductasePantetheineNADPHNADPNADPHNADPFatty acidsynthase-ketoacyl11Cholesterol de novo synthesis IIsoprenyl formationAcetoacetyl-CoAThiolaseHMG-CoAsynthaseMevalonate3-Hydroxy-3-methylglutaryl-CoAMevalonatekinasePhosphomevalonatekinaseMevalonate-5-pyrophosphodecarboxylase5-Phosphomevalonate5-Pyrophosphomevalonate3-Isopentenylpyrophosphate3,3-DimethylalylpyrophosphateIsomeraseHMG-CoAreductase2 NADPH2 NADPATPADPADPATPADPATPCholesterol de novo synthesis 12CholesterolBiosynthesis of bile acids7-HydroxycholesterolSterol 7 hydroxylaseCYP7A17-Hydroxy-4-cholesten-3-one3-Hydroxystroid dehydrogenaseHSD3B77,12-Dihydroxy-4-cholesten-3-oneSterol-12-hydroxylaseCYP8B15-Cholestan-3,7,12-TriolOxosteroid-5-reductaseAKR1D1Sterol 27-hydroxylaseCYP27A13,7,12-Trihydroxy-5-cholestanoic acidCholic acidAKR1D1AKR1C45-Cholestan-3,7-diol12-Deoxycholic acid3-Hydroxy-steroid dehydrogenaseAKR1C4CholesterolBiosynthesis of bil13Biosynthesis of RNARNAPolymeraseBiosynthesis of RNARNAPolymer14Biosynthesis of DNADNAPolymeraseBiosynthesis of DNADNAPolymer15-oxidationMitochondrial matrixIntermembranespacePalmitoyl carnitinePalmitoyl carnitinePalmitoyl CoAPalmitoyl CoATrans 2 enolopalmitoyl CoA3-Hydroxypalmitoyl CoA3-Ketopalmitoyl CoAMyristoyl CoAAcetyl-CoAPalmitoyl CoAPalmitatePalmitoyl CoAligaseATPAMPCarnitineacyltransferase ICarnitineCarnitineacyltransferase IIAcyl CoAdehydrogenaseEnoyl CoAhydratase3-Hydroxyacyl CoAdehydrogenaseCitric AcidCycleFADFADH2NADNADHCytoplasmH2O-oxidationMitochondrial matri16Myo-Inositol-3-phosphateIns(3)P1Phospho-myo-inositol Toolkit 2Myo-Inositol-3,4-diphosphateIns(3,4)P2Myo-Inositol-3,6-diphosphateIns(3,6)P2Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3Myo-Inositol-3,4,5,6-tetraphosphateIns(3,4,5,6)P4Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4Myo-Inositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1,3,4,5,6)P5Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5Myo-Inositol-1,2,3,4,5,6-hexaphosphateIns(1,2,3,4,5,6)P6 (Phytic acid)Myo-Inositol-3-phosphateIns(317Myo-Inositol-3-phosphateIns(3)P1Phospho-myo-inositol ToolkitMyo-Inositol-3,4-diphosphateIns(3,4)P2Myo-Inositol-3,6-diphosphateIns(3,6)P2Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3Myo-Inositol-3,4,5,6-tetraphosphateIns(3,4,5,6)P4Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4Myo-Inositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1,3,4,5,6)P5Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5Myo-Inositol-1,2,3,4,5,6-hexaphosphateIns(1,2,3,4,5,6)P6 (Phytic acid)Myo-Inositol-3-phosphateIns(318GlycogenGlycogeninGlycogenGlycogenin198-oxoguanineA common DNA lesions Toolkit IO6-methylguanineUracilThymine glycol1,N6-ethenoadenine3-methyladenineCyclobutane pyrimidine dimersPhotoadduct8-oxoguanineA common DNA lesio20RiboseGlucuronateArabinoseFructoseGlucoseGalactoseMannoseFucoseSialic AcidInositolD-AbequoseL-RhamnoseA carbohydrate toolkitRiboseGlucuronateArabinoseFruc21PGH2TXA2TXB2Bioactive lipids-ProstanoidsPGD2PGJ2PGE2PGA2PGB2PGF2PGF-MPGI26-keto-PGF1 PGH2TXA2TXB2Bioactive lipids-22Bioactive lipids-Leukotrienes5-HETELTA4LTB4LTC4LTD4LTE420-OH-LTE4LXA4LTB4LTF4LXALXBBioactive lipids-Leukotriene23Bioactive lipidsArachidonateCeramideSphingosine-1-phosphatePAFPhosphatidic acidAnandamide 2-Arachidonoylglycerol Bioactive lipidsArachidonateCe24 qGTP NO mediated attenuation of adrenergic Gq signaling in VSMC qGDP PLC qGTPCasGCcGMPpGCANPSERCABKCaPKGPKCMLCKmyosinmyosinmyosinPPCaM MLCKCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaK+CaCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaM Ca PA1,4,5-InsPqGTPNO mediated attenuat25 SGTP RGS function and regulation SGDP ACPKAcAMP cAMPcAMPcAMPcAMPcAMPcAMPAC SGTPLigandLigandRGSRGSRGS14-3-3RGSP14-3-3PcAMPcAMPcAMPTNF SGTPRGS function and regul26Modular Structure of Cytokine ReceptorsIFN R2515 aaIFN R1 530 aaIL-6R 449 aaIL-11R 400 aaIL-10R1 557 aaIL-10R2325 aaSTATbindingIL-13R 1 427 aaIL-4R 825 aaFNIIIFNIIICCCCWS-WS FNIIIFNIIIFNIIIFNIIIFNIIIFNIIIFNIIIFNIIIFNIIIFNIIISOCSgp130 896 aaSTATbindingTyk2Jak1INF R1 489 aaSTATbindingFNIIIFNIIIJak1IL-22R1 574 aaSTATbindingFNIIIFNIIIJak1Jak1INF R2 332 aaFNIIIFNIIIJak2STATbindingTyk2Jak1Jak2Jak1IL-13R 2 380 aaFNIIIFNIIICCCCWS-WS FNIIIFNIIICCCCWS-WS FNIIIFNIIIFNIIISTATbindingSTATbindingIgIgIgIgOSM-R952 aaJak3FNIIIFNIIIFNIIIFNIIISTATbindingIgFNIIIFNIIICCCCWS-WS FNIIIFNIIICCCCWS-WS FNIIIFNIIICCCCWS-WS LIF-R 1053 aaJak3FNIIIFNIIIFNIIISTATbindingIgFNIIIWS-WS FNIIIFNIIICCCCWS-WS STATbindingSTATbindingSHPFNIIICCCCSTATbindingSTATbindingTyk2FNIIIFNIIICCCCWS-WS IgSOCSSHPFNIIIFNIIICCCCWS-WS IgJak3 c 349 aaIRSFNIIIFNIIICCCCWS-WS IgJak3FNIIIFNIIIFNIIIFNIIIFNIIICCCCWS-WS IL-12 662 aaFNIIIWS-WS SOCSIRSSTATbindingIgTyk2Fibronectintype III domainIg-likeIRS-bindingFibronectintype III domainwith cysteinesSOCS-bindingSTAT-bindingJAK/TYK-bindingFibronectintype III domainwith WSXWSSHP-bindingSHPModular Structure of Cytokine 27Adaptor proteins-selected(human)SAMdomainSrc-homology 3 domainSH3SH2Src-homology 2 domainGRB2PTBdomainPTBCysteine-richregion 1Calponinhomology domainC-1CalponinSproutySprouty domain1217SH2SH3SH31276SH2SH3SLAP1304SH2SH3SH31330SH2SH3SH3CrkGADS(GRID)1377SH2SH3SH3SH3Nck11456SH2SproutyBLNKRho-GEFRho-GEF homologydomainVav-11533SH2SAMSLP-761584SH2PTBSHC-11632SH2PHAPS1845SH2SH3SH3PHC-1Rho-GEFCalponinSAMPHPlekstrin homologydomainAdaptor proteins-selected(h28Protein separation using SDS-PAGE(Laemmli system)StackinggelResolvinggel1.Apply protein/dye samples into polyacrylamide gel wells2.Run the electrophoresis until dye reaches the end of the gel3.Remove the gel from the apparatus and stain for proteinsProtein separation using SDS-P29A Column Chromatography ToolkitA Column Chromatography Toolki30Embryonic stem cellsEgg donorSperm donorEgg fertilizationCulture fertilized cells forseveral cell divisionsDissociate cell clusterfor subculturing andfurther utilizationFreeze in N2 forlong-term storageUse for In VitrofertilizationPotential use fortherapeutic cloningEmbryonic stem cellsEgg donorS31Tet rAmp rOripBR3224361bpOripBluescript IIAmp rLacIMCSLacZf1 OriOripUC18Amp rMCSLacZCommon Cloning VectorsOripCR2.13929bpAmp rKanLacZf1 OriM13(-)APCRAT7M13(+)OripGEM 11Zf3221bpAmp rLacZf1 OriT7 SfiI-SacI-EcoRI-SalI-Xho-BamHI-ApaI-XbaI-NotI-SphI-NsiI-HindIII SP6OripGEM 9Zf2912bpAmp rLacZf1 OriSP6 NsiI-HindIII-XbaI-EcoRI-SalI-SacI T7Tet rAmp rOripBR3224361bpOrip32Metabolism of capecitabine5-FU5-Fluorouridine5-FUMP5-FdUMPUridine phosphorylase5-FluorodeoxyuridineThymidine phosphorylaseOrotatephosphoribosyltransferaseThymidine kinase5-FUTP5-FdUTPRNADNA5-nucleotidase5-nucleotidaseUridine kinaseATPADPATPADPPRPPCapecitabineXeloda(prodrug)Carboxyl-esterase5-Fluoro-5-deoxycytidineCytidinedeaminaseThymidine phosphorylase5-Fluoro-5-deoxyuridinePharmacologicallyactive compoundMetabolism of capecitabine5-FU33PertussistoxinNADADPCholeratoxin ACATPcAMPAC SGTPLigandPharmacological modulation of cAMPLigandLigandLigand iGDP SGDP iGTP iGTPNADADPForskolinRolipramAMPPDE SGTPPertussistoxinNADADPCholerat34Mechanism ofPenicillin(inhibition of crosslinkingpeptidoglycan)Tetra-peptidePenta-glycineGlycopeptidetranspeptidasePenicillinMechanism ofPenicillin(inhib3540SInhibition of translation byRapamycin(Sirolimus)Growth Factor ReceptorsIP3KPDKPKBmTOR40S30SeIF4GeIF4EPABPeIF4A4E-BP1eIF4EPP4E-BP1PPPPPS6KPPPPPRibosomalproteinseEF2eEF2PP40S30SeEF2kinase CaMeIF2RapamycinFKBP-1240SInhibition of translation36TMPMechanism of 5-fluorouracil(5-FU)deoxyUMPThymidylatesynthaseTTP5-FUCarac,EfudexDNA synthesis5-Fluorouridine5-FUMP5-FdUMPUridine phosphorylaseUridine kinase5-FluorodeoxyuridineThymidine phosphorylaseOrotatephosphoribosyltransferaseThymidine kinaseN5,N10-methylene-THFDHFTMPMechanism of 5-fluorouracil37The Life cycle of Plasmodium spp.Infected mosquito bitereleases sporozoitesinto human bloodBloodvesselEndotheliumHepatocytesKupffer cellsMerozoiteSporozoiteTrophozoiteSchizontMacrogametocyteMicrogametocyte1234Mosquito bitetakes up macrogametocytesand microgametocytesOocystGametogenesisMosquito gutThe Life cycle of Plasmodium s38The Life cycle of HeartwormDirofilaria immitisMicrofilariaeLarvae14 daysMicrofilariaeLarvae14 daysLarvaeAdult worm6monthsLarvaeAdult worm8monthsMicrofilariaeMicrofilariaeThe Life cycle of HeartwormDi39Virus envelopes IIIVirus envelopes III40HIV envelopeHIV envelope41Bacteria&Bacteriophage ToolkitBacilliCoccispirillaBacteriophage Bam35Bacteriophage T4Bacteriophage T2Bacteria&Bacteriophage Toolk42AdenovirusAdenovirus43Citric acid cycleCitrateSuccinyl-CoAsynthetaseAconitaseCitrate synthaseSuccinatedehydrogenaseGTPGDPOxaloacetateAconitaseIsocitratedehydrogenaseIsocitratedehydrogenase-KetoglutaratedehydrogenaseSuccinyl-CoA-KetoglutarateMalatedehydrogenaseFumarasecis-AconitateIsocitrateOxalosuccinateSuccinateFumarateMalateFADFADH2NADNADHCO2NADNADHCO2NADNADHH2OH2OH2OGlyoxylate cycleGlyoxylateGlyoxylate cycleH2OFattyacidsCitric acid cycleCitrateSuccin441 2 3 4 5 6 7 8 9 10 11 12 1314 15 16 17 18 19 20 21 22 X YHuman chromosomes1 2 3 45分子生物学之PPT课件素材精编46分子生物学之PPT课件素材精编47分子生物学之PPT课件素材精编48分子生物学之PPT课件素材精编49Needle tip toolkitNeedle tip toolkit50A DNA Toolkit 3A DNA Toolkit 351RNA ToolkitMet-tRNARNA ToolkitMet-tRNA52A DNA Toolkit 4A DNA Toolkit 453分子生物学之PPT课件素材精编54AstrocyteNeuronAstrocyteNeuron55Neutrophil Eosinophil BasophilMesenchymal cellSmooth muscle cellsEpithelial cellsMacrophageDendritic cellT-cellErythrocyteB-cellNeutrophil Eosin56分子生物学之PPT课件素材精编57分子生物学之PPT课件素材精编58Antibody Toolkit(IgG)Antibody Toolkit(IgG)59 helixVimentinActin and myosinCoiled-coilMicrotubule6 nm10 nm25 nm helixVimentinActin and myos60分子生物学之PPT课件素材精编61分子生物学之PPT课件素材精编62分子生物学之PPT课件素材精编63Nucleosomes 2Nucleosomes 264分子生物学之PPT课件素材精编65ACACDDR1DDR1DDR176123454356217CD45PTPACACDDR1DDR1DDR17612345435621766
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