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1进入NCBINCBI Resources (yl HowTo %NCBIAll Databases TNational Center forBiotechnology InformationNCBI HomeWelcome to NCBIResource List (A-Z) All ResourcesChemicals & BioassaysData & SoftwareThe National Center for Biotechnology Information advances science and health by providing access to biomedical and genomic information.About 也e NCBI | hlission | 0已nizsttion | Rose日ch | NCEI New;DNA & RNADomains & StructuresGenes & ExpressionGenetics & MedicineGenomes & MapsGet Started Fools: Analyze data using NCBI software Dcrwnlo日怡 Get NCBI data or softv/are Hciw-Trsi Learn how to accomptfsh specific tasks at NCBI Submit data to GenBank or other NCBI databases2.选择:Nucleotide，输入物种拉丁名如Poria cocos3. 查询得如下:2 NCBI Resources 1HowTo NucleotideNucleotidetPoria cocosS已he search Limits AdvancedDisul SEttinqs: Q Summary, 20 per page, Sorted by Default order 統 Fourrd 643 nudeotide sequences. Nucleotide (457) EST (186)Results: 1 to 20 of 457 眈Poria cqgos strain KAMP Mo. 7233 28Sibosomal RNA gene, partigil1 2,821 bp linear DNAAccession: EF3&7600.1 Gl: 126508724G已门日日门kFASTA G日uhic呂 Ft巳I日td S已口u巳nc巳sPdi怡 coco导 stajn KANP No. 了067 28S 冋boomal RNA gmnm. parti对 smqumnue2 2;823 bp linear DNAAccession: EF397599.1 Gl: 126508723门kFASTA G且ohics民吕ltEcI Seciu巳门c巳sFo怡 cocos strain K1 28S ribosonn创 RNA gene, parti创 sequenue3-2,865 bp linear DNAAccession: EF397598.1 Gl: 126508722G巳门日日门kFAST A G日uhics 民巳伯Iscl Seciij巳门ces4. 选择该标题For怡 cocos strain KAMP No. 7233 28S ribosomal RNA genm partial sequmnum 1-2,821 bp linear DNAAccession: EF397600.1 Gl: 126508724G巳riB日门kPASTA G日Dhics R巳I日tEd Secju巳ncES5. 进入之后就是一下画面,Di导play Settings: Q GenSankPoria cocos strain KANP No. 7233 28S ribosomal RNAsequenceGenBank: EF397600.1FASTA GraphicsGoto: ElLOCUSEF3976002821 bp DNA linear PLN 13-SEP-2011DEFINITIONForj a iiQGos strain KANP No. 7233, 28S ribosomal RNA gene partialsequence.ACCESSIONEF397e0OVERSIONEF3976QO： 1GI:126508724KEYWORDSSOURCEWolfiporiaJq.cosORGANISKWcilfiDcxri且guc：日Eukaryota; Fungi; Dikarya; Basidioinyeota; Agaricoirycotina; AgaricomyGetePolyporales; Wolfiporia.REFERENCE 1 (bases 1 to -2821)6.点击下图中的FASTA,Poria cocos strain KANF sequenceGenBank: EF397600.1FAST A G 闻 hi%7.进入之后就是以下内容，也是最终结果，其中，物种名为：Poria cocos ,登录号为：EF397600,基因序列为:GACCTCAGPoria cocos strain KANP No, 7233 28S ribosomal RNA gene, partial sequenceGenBank: EF397600.1GenE 日 nkGr也 ptiicsgi 126508724|gb:EF397600. 1| Poria cocos strain KANP No. 7233 28S ribosomal RNA gene partialsequenceGACCTCAGATCAGGCAGGACTACCCGCCGAACTTAAGCATATCAATAAGCGGAGGAAMGAMCTAACM GGATTCCCCTAGTMCTGCGAGTGMGAGGGAAGAGCTCACATTTAAAATCTGGCGGTCTGGCGGCCGTa CGAGTTGTAGCGTGGAGAAGCGTCGTCTGCGCCGGACgCGTGTACMGTCTC&TGGAACGGAGCGTCGTAGAGGGTGATMTCCCGTCGTCGACACGGACCTCCGGTTAGCTATGCGAGGCGCTCTCGAAGAGTCGAGTTGTTTGGGATGCAGCTCAGTGGGTGGTGATTCCATCTAGCTATACAGGCGAGAGACCGATAGCGGACAAGTACCGTGAGGGAMGATGAAAAGCACTCTGGAAAGAGAGTTAMCAGCACGTGAMTCGCTGAGAGGGAMCGCTTGMGTCAGTCGCGTCGGCGTTAGGAGGTCAGCCTTCCCGGGATAATAACCGTGGGGCGCACTgtCCCGTCGACGGGCCAGCATCGATTTCGACCGCCGGAGAAGGGCTCGGGGMCGTGGCACCTCC-GGGTGTGTTATAGCCCCCTGTCGTATGCGGCGGTCGGGATCGAGGTTTTTACATGTTCGCAGCACGCCTTTTTGGCCGGGGCCGTTCGCGGTCCACGTCCGTGCTTAGGATGCTGGCGTMTGGCTTTRAACGACCCGTGTTGAAACACGGACCAAGGAGTCCAACTCGCCTGCGAGTGTTTGGGTGGAAAACCJCGAGCGCGTMCGAAA8.前面选择Protein,输入物种名如Poria cocos,搜索出来如下内容,Disula* Settings: Summary, 20 per page, Sorted by Defauft orderPage 匚Results: 1 to 20 of 74q伽nmsyl-diphosphatm synthasm Wolfipor怡 cocos1361 aa proteinAccession: AFR13039.1 Gl: 403065235G巳门FASTA GranhicsR巳卜日td S巳ciu巳nc巳sL 1 fammsyldipliosphatQ synthasm (Wolfipor他 coco歸 2361 aa proteinAccession: AFR13038 1 G: 403065233G已nF1 巳Dt FASTAG日ohicsRelat巳ci S已口uenc巳snualpnA vnihAA IWnlfinnria rnrnsl9.选择标题，如选择2,|famesW-diphosp打atm synthase Wolfipori自 cocosj2361 aa proteinAccession: AFR13038.1 Gl: 403065233G吕门PeotFASTA GrmDhics Ft巳I日S巳due门ces10.进入之后为如下内容。farnesyl-diphosphate synthase Wolfiporia cocosGenBank: AFR13038.1FAWTA GraphicsGoto: QLOCUSAFR1303361 aalinear PEN 09-OCT-2012DEFINITION ACCESSION VERSION DBSOURCE KEYWORDS SOURCEfarnesyl-diphosphate synthase .Wolfiporia co：os.AFR13038AFR13033.1 GI:403035233accession .TN652835. 1View conserved domai using CD-search.ORGAN REFERENCEAUTHORSWolfj.poria cocosWdlfj.DCiri且 cciccimEukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;AgariOinyfetes; Polyporales; Wolfiporia.1 (residues 1 to 361)Wangj J., GuOj L., Werij J., Guan吕S. and Lin, J.11.点击 FASTA,farnesybdiphosphate synthase Wolfiporia cocosGenBank: AFR13038.1FASTA G 田 uhics12.进入之后为如下内容，其中物种名为：Wolfiporia cocos ，登陆号为：AFR13038.1,蛋白质序列为：MSSSSAKVQKRQKFEAVFPVLRDEL。farnesyl-diphosphate synthase Wolfiporia cocosGenBank: AFR13038.1gi j 403065233 | gb ; AFR13038. 1 farnesyl-diphosphate synthase iWolf.iporia cocosMSSS SAKVQKRQKFEAVFPVLRDELVAHLAAQGMPEDAKEWYTKNLDYNVPGGKLNRGLAWDTVEILKG RSLTDEEYFKAALLGWCT7EFLQAFFLVSDDKMDQ SVTRRGQPCYYRLERWI AW SFKLEMAIYYLIKSHFRSDPCYGYLIELFHETTYQTEIGQLIDLITAPEDHVDLTKF SLEKYKLIVIYKTAYYSFYLPVAAAMYMCGIPHSASNDPYELARSILIPLGEYFQVQDDFLDFAGTPEQIGKIGTDIIDNKCSWCINTALAHATPE QRTVLDANYGRKDAAAEARVKALYHEMDIPKLYEQYEEAAYKRIMGLIETIPAEPVAPQGQWLRREVFV SFLQKIYKRTK
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