不同浓度SDS-PAGE胶

SytiO buHefMolnr rWtio pf ncrfnmi-rlzbiGrfnmLriR iy 23:1.加入文库w获取下载特权不同淞度的 DS- PAG E胶分离蛋白的范围Sepsrstion ranges o/praft?/fls Jn danoturing SDS-PAGE*Rnragpfli 肾卩nmliign of firpt 将ns画蛋白质SDS聚丙烯酰胺凝胶电泳配方A creek44 上棒于 2016-06-15 5.0分隔刊9%的対O 134612 牺优质文档分离胶；一块I交配5mLResolving Gel6%8%10%12%15%5mLWimL5mLlOmL5mLlOmL5mLlOmL5mLlOmLHz02.6mL5.3mL2.3mL4.6mL1.9mL4m L1.6mL3.3mLl.lmL2.4mL30% mixImL2imL1.3mL2.7mL1.7mL3.3mL2mL4mL2.5mL5mL1.5M Tris pH 8.81.3mL2.5mL1.3mL2.5mL1.3mL2.5mL1.3mL2.5mL13mL2.5imL10% SDS50uLlOOuL50uLlOOuL50uLlOOuL50uLlOOuL50uLlOOuL10% APS50uLlOOuL50uLIOOljL50uLlOOuL50uLlOOuL50uLlOOuLTEMED4uL8uL3uL6uL2uL4uL2uL4uL2uL4uL每一块胶需要约4泌，留岀约2-3cm的距离，用水封口，防止空气接触。室温放萱30伽后，可以看到构口胶的界 面重新岀现。弃去上层水，倒入擬缩胶。优质文档Stacking Gell5%2imL5mLH201.46mL3.6mL30% mix330uL830uL1.5IVITris pH6.8170uL420uL10% SDS20uL50uL10% AP20uL50uLTEMED2uLSuL过硫g麦镀1称取IgAPS2加入lOmL去离子水3储存于比可使用两周时间,过期失去傕化作用。5:_ Tris-Glyciine Buffer（SDS-PAGE 电泳缓冲液）Tris15.1gGlly94gSDS5g2加入gOOmL去离子水，搅拌溶解3定容至1L,室温保存。5*SDS-PAGE Loading Buffer1.10ml离心管IM Tris-HClpH6.8）1.25m LSDSQ.5gBPB25 mg甘油2.5 ml2加去离子水潛賄定容至5mL3小份（5QQUL/份）分装后，于室温保存4使用前将25uL的2-ME加到每小份中5加A 2-ME的Loading Buffer可在室温下保存一月左右