血涂片制备染色及白细胞形态观察【-】课件

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,血涂片制备染色及白细胞形态观察,临床检验基础与输血教研室,血涂片制备染色及白细胞形态观察临床检验基础与输血教研室,1,根据不同的细胞种类及细胞的不同成分，对酸性及碱性染料的结合能力不同，而使各种细胞呈现出各自的特点。,掌握血涂片的制备和染色方法及白细胞的正常形态,原理,目的,根据不同的细胞种类及细胞的不同成分，对酸性及碱性染料的结合能,2,器材,载玻片、推片、洗耳球、显微镜、微量吸管、香柏油、乙醇-乙醚溶液、拭镜纸,试剂,瑞氏染液、磷酸缓冲液,标本,抗凝血,器材 载玻片、推片、洗耳球、显微镜、微量吸管、香柏油、乙醇,3,血 涂 片制 备,推片,载玻片,血液,将推片匀速向前，勿停顿。涂片的厚薄取决于速度和角度：快、大(厚)。慢、小（薄）,一张满意的血涂片应厚薄均匀,头 体 尾鲜明,涂片干燥后用铅笔在血涂片的头,部写上姓名和编号,李四,B,1 2 3,血 涂 片制 备推片载玻片血液将推片匀速向前，勿停顿。涂片的,4,血 涂 片 染色,勿冲洗再加,瑞氏染色液5-8滴后将液和液充分混匀静置10分钟,直接流水冲洗3-5分钟,（切勿先倒掉染液）,涂片经水洗干燥后用油镜,分类计数100个白细胞,李四123,涂片干燥后加瑞氏染色液5-8滴覆盖整个血膜1分钟,血 涂 片 染色勿冲洗再加瑞氏染色液5-8滴后将液和液,5,血涂片的观察,低倍镜观察,1.染色情况(满意、偏酸、偏碱）,2.细胞分布情况,油镜下分类计数：体尾交界处。,油镜分类时应按一定走向，不要重复计数,。,李四123,血涂片的观察低倍镜观察李四123,6,中性杆状核粒细胞,中性杆状核粒细胞,7,中性分叶核粒细胞,中性分叶核粒细胞,8,嗜酸性粒细胞,嗜酸性粒细胞,9,嗜碱性粒细胞,嗜碱性粒细胞,10,单核细胞,单核细胞,11,1.本次实验的关键环节是什么？,2.每人分类的结果与本组其他人使用同一标本的同学是否一致，分析原因。,分析讨论,分析讨论,12,K+oN3sR6vVazYdC$hG-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8xXcB#fF&jI)mM1qP4tT8AZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT8yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vU9zYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT8yXcB#gF&jI)nM1qP4uT8A#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jI)mL1qP4tS8xWbA#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jI)mL1qP4tS8xWbA#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uT9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbA$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rR6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qQ5uT8xXcB#fE&jI)mL1qP4tT8xWbA#fE%iH)mL0pP4tS7wWbAZeD%iH(lK0pO3sS7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rR6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qQ5uT8xXcB#fE&jI)mL4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+pO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-oN2rQ5vU9yXcC!gF&jJ-nM1rQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0qP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+pO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1rQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0qP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-nN2rQ5vU9y#gF&jI-nM1qP5uT8xWcB#fE%jI)mL1qP4tS8xWbAZfE%iH(mL0pO4tS7wVbAZeD$iH(lK0pO3sR7wVazYeD$hG*lK+oN3sR6vUazYdC!hG*kJ+oN2rQ6vU9yXdC!gF&kJ-nM2rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL1qP4tS8xWbAZfE%iH(mL0pO4tS7wVbAZeD$iH(lK0pO3sR7wVazYeD$hG*lK+oN3sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM2rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB%iI)m