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,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,穴位肥大细胞及其颗粒物,在针刺镇痛中的作用,针灸经络研究回顾与展望国际学术研讨会,Outline,穴位肥大细胞在针刺镇痛效应中的作用,穴位胶原纤维在针刺镇痛效应中的作用,穴位注射组胺的镇痛效应,手针和电针穴位信号的启动机制,Mast cells in acupoints and sideward,Comparison of Number of MCs in Zhushanli and non-acupoints of Rat,Group Name,MCs Number,Skin,Muscle,Acupoint,（,n,6,）,34.22.5,12.41.4,Non-acupoint,（,n,6,）,22.11.7*,8.51.0*,THE JOURNAL OF SCIENCE&HEALING,2008,，,4,（,3,），,170-177,1.The relationship betweenmast cell function and acupuncture effect,Animal model,：,AA,(acute arthritis),Rat,Complete Freunds adjuvant,Acupoint selected,：,Zushanli (,ST36,),D,ST,36,C,1.The relationship betweenmast cell function and acupuncture effect,Treat effect,：,Ease pain,，,Pain threshold,1.The relationship betweenmast cell function and acupuncture effect,The degranulation of mast cells,SD Rats,Tail Flick Model,Anatomy Position of Zusanli,Acupuncture,Injection drugs for prevent degranulation of MC,A,B,D,E,ST,36,C,Pain threshold of rat when shield mast cell degranulation,1.The relationship betweenmast cell function and acupuncture effect,ID,Group Name,组别,Average SEM(%),A,Control,正常对照组,1.90.1,B1,Acupuncture at ST36,穴位针刺组,11.60.6*,B2,Acupuncture at Nearby-ST36,旁开针刺组,8.90.3*,C1,Injection of DSCG at ST36,色甘酸钠注射组,1.70.1*,C2,Injection of NaCl at ST36,生理盐水注射组,1.40.1*,D1,Acupuncture after DSCG at ST36,色甘酸钠注射针刺组,6.20.4*,D2,Acupuncture after NaCl at ST36,生理盐水注射针刺组,8.90.4*,D3,Acupuncture opposite side after DSCG,色甘酸钠注射对侧针刺组,9.30.1*,各组别大鼠痛阈变化率峰值比较（,20,分钟时）,(n=6),Note:*P0.05 vs.Group A;,P0.01,P0.05 vs.Group B1;,P0.01,P0.05 vs.Group D1,1.The relationship betweenmast cell function and acupuncture effect,Group Name,组别,MC Degranulation Ratio,(Average SEM)(,),Skin,Muscle,Control,正常对照组,29.21.2,26.31.8,Acupuncture,穴位针刺组,48.93.9*,32.23.1*,*,Acupuncture after DSCG,色甘酸钠注射针刺组,33.72.8,28.52.3,Acupuncture after NaCl,生理盐水注射针刺组,50.42.5*,29.72.3,穴区组织肥大细胞脱颗粒率统计,(n=6),Note:*P0.01,*P0.05 vs.Control Group;,P0.01 vs.Acupuncture Group.,Results from Experiment,Staining of Mast Cells in Acupoint on Rats,Muscle,Skin,Before Acupuncture,After Acupuncture,Degranulation,of cells,After SDCG Stabilized,THE JOURNAL OF SCIENCE&HEALING,2008,，,4,（,3,），,170-177,What is the key factor to degranulation,The acupuncture effect is positive relationship to the degranulation of mast cell at acupoint.,What is key factor conduce the degranulation of mast cell during acupuncture manipulation?,Our focus is on the collagen at acupoint.,2024/11/14,13,To observe the function of collagen fibers in MA analgesia.,7,Animal groups setting:,normal group(N):Normal healthy animal,control group(M):Animal with AA,injection of collagenase at Zusanli(ST36)group(C),manual acupuncture group with rotation manipulation(R),or lift-thrust manipulation(L),rotation group with collagenase pretreatment to destroy,Zusanli(ST36)collagen fibers(CR),lift-thrust group with collagenase pretreatment to destroy,Zusanli(ST36)collagen fibers(CL),lift-thrust group pretreated with collagenase to destroy,Dubi(ST35)collagen fibers(CDL),(R),(L),2024/11/14,14,Analgesia effects observed in different groups,Group,n,Pain Threshold(PT),4d Before MA(s),After MA(s),Change Rate(%),MC Degranulation Ratio,(%),N,8,9.190.65,-,-,10.62.6,M,8,5.610.48*,-,-,14.15.9,C,8,5.900.24*,5.590.52,-5.1,11.94.7,R,8,5.440.37*,7.520.65,38.5,75.74.4,L,8,6.130.82*,8.501.56,39.1,76.95.9,CR,8,5.270.62*,5.390.57,3.3,10.42.4,CL,8,6.050.43*,6.260.79,3.8,12.42.3,注：较正常组痛阈：*,P0.01,；较处理前痛阈：,P0.05,；较捻转组,(R),痛阈变化率：,P0.05,；较捻转组,(L),痛阈变化率：,P0.05,；较正常组或模型组,:,P0.05,；较捻转组或提插组,:,P0.05,。,2024/11/14,15,Analgesia effects observed in different groups,Group,n,Pain Threshold(PT),4d Before MA(s),After MA(s),Change Rate(%),MC Degranulation Ratio,(%),N,8,9.190.65,-,-,10.62.6,M,8,5.610.48*,-,-,14.15.9,C,8,5.900.24*,5.590.52,-5.1,11.94.7,R,8,5.440.37*,7.520.65,38.5,75.74.4,L,8,6.130.82*,8.501.56,39.1,76.95.9,CR,8,5.270.62*,5.390.57,3.3,10.42.4,CL,8,6.050.43*,6.260.79,3.8,12.42.3,注：较正常组痛阈：*,P0.01,；较处理前痛阈：,P0.05,；较捻转组,(R),痛阈变化率：,P0.05,；较捻转组,(L),痛阈变化率：,P0.05,；较正常组或模型组,:,P0.05,；较捻转组或提插组,:,P0.05,。,2024/11/14,16,Analgesia effects observed in different groups,Group,n,Pain Threshold(PT),4d Before MA(s),After MA(s),Change Rate(%),MC Degranulation Ratio,(%),N,8,9.190.65,-,-,10.62.6,M,8,5.610.48*,-,-,14.15.9,C,8,5.900.24*,5.590.52,-5.1,11.94.7,R,8,5.440.37*,7.520.65,38.5,75.74.4,L,8,6.130.82*,8.501.56,39.1,76.95.9,CR,8,5.270.62*,5.390.57,3.3,10.42.4,CL,8,6.050.43*,6.260.79,3.8,12.42.3,注：较正常组痛阈：*,P0.01,；较处理前痛阈：,P0.05,；较捻转组,(R),痛阈变化率：,P0.05,；较捻转组,(L),痛阈变化率：,P0.05,；较正常组或模型组,:,P0.05,；较捻转组或提插组,:,P0.05,。,collagen fibers of skin and muscle,Mast cells in skin,Group,Normal,Group,Inject,Control,collagen fibers of skin and muscle,Mast cells in skin,Connective Tissue Research,2008,，,50,（,2,）,:110-120,Acupunc.,whirling,Acupunc.lift and thrust,collagen fibers of skin and muscle,Mast cells in skin,collagen fibers of skin and muscle,Mast cells in sk
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