穴位肥大细胞及其颗粒物在针刺镇痛中的作用

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,2,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,1,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,纪念安徽针灸经络研究,50,周年,穴位肥大细胞及其颗粒物在针刺镇痛中的作用,针灸经络研究回顾与展望国际学术研讨会,Outline,穴位肥大细胞在针刺镇痛效应中的作用,穴位胶原纤维在针刺镇痛效应中的作用,穴位注射组胺的镇痛效应,手针和电针穴位信号的启动机制,Mast cells in acupoints and sideward,Comparison of Number of MCs in Zhushanli and non-acupoints of Rat,Group Name,MCs Number,Skin,Muscle,Acupoint,（,n,6,）,34.22.5,12.41.4,Non-acupoint,（,n,6,）,22.11.7*,8.51.0*,THE JOURNAL OF SCIENCE & HEALING, 2008,，,4,（,3,），,170-177,1. The relationship betweenmast cell function and acupuncture effect,Animal model,：,AA,(acute arthritis),Rat,Complete Freunds adjuvant,Acupoint selected,：,Zushanli (,ST36,),D,ST,36,C,1. The relationship betweenmast cell function and acupuncture effect,Treat effect,：,Ease pain,，,Pain threshold,1. The relationship betweenmast cell function and acupuncture effect,The degranulation of mast cells,SD Rats,Tail Flick Model,Anatomy Position of Zusanli,Acupuncture,Injection drugs for prevent degranulation of MC,A,B,D,E,ST,36,C,Pain threshold of rat when shield mast cell degranulation,1. The relationship betweenmast cell function and acupuncture effect,ID,Group Name,组别,Average SEM (%),A,Control,正常对照组,1.90.1,B1,Acupuncture at ST36,穴位针刺组,11.60.6*,B2,Acupuncture at Nearby-ST36,旁开针刺组,8.90.3*,C1,Injection of DSCG at ST36,色甘酸钠注射组,1.70.1*,C2,Injection of NaCl at ST36,生理盐水注射组,1.40.1*,D1,Acupuncture after DSCG at ST36,色甘酸钠注射针刺组,6.20.4*,D2,Acupuncture after NaCl at ST36,生理盐水注射针刺组,8.90.4*,D3,Acupuncture opposite side after DSCG,色甘酸钠注射对侧针刺组,9.30.1*,各组别大鼠痛阈变化率峰值比较（,20,分钟时）,(n=6),Note: *P0.05 vs. Group A;,P0.01,P0.05 vs. Group B1;,P0.01,P0.05 vs. Group D1,1. The relationship betweenmast cell function and acupuncture effect,Group Name,组别,MC Degranulation Ratio (Average SEM) (),Skin,Muscle,Control,正常对照组,29.21.2,26.31.8,Acupuncture,穴位针刺组,48.93.9*,32.23.1*,*,Acupuncture after DSCG,色甘酸钠注射针刺组,33.72.8,28.52.3,Acupuncture after NaCl,生理盐水注射针刺组,50.42.5*,29.72.3,穴区组织肥大细胞脱颗粒率统计,(n=6),Note: *P0.01, *P0.05 vs. Control Group;,P0.01 vs. Acupuncture Group.,Results from Experiment,Staining of Mast Cells in Acupoint on Rats,Muscle,Skin,Before Acupuncture,After Acupuncture,Degranulation,of cells,After SDCG Stabilized,THE JOURNAL OF SCIENCE & HEALING, 2008,，,4,（,3,），,170-177,What is the key factor to degranulation,The acupuncture effect is positive relationship to the degranulation of mast cell at acupoint.,What is key factor conduce the degranulation of mast cell during acupuncture manipulation ?,Our focus is on the collagen at acupoint.,2024/9/21,13,To observe the function of collagen fibers in MA analgesia.,7,Animal groups setting:,normal group (N): Normal healthy animal,control group (M):Animal with AA,injection of collagenase at Zusanli(ST36) group (C),manual acupuncture group with rotation manipulation (R),or lift-thrust manipulation (L),rotation group with collagenase pretreatment to destroy,Zusanli(ST36) collagen fibers (CR) ,lift-thrust group with collagenase pretreatment to destroy,Zusanli(ST36) collagen fibers (CL) ,lift-thrust group pretreated with collagenase to destroy,Dubi(ST35) collagen fibers (CDL),(R),(L),2024/9/21,14,Analgesia effects observed in different groups,Group,n,Pain Threshold(PT),4d Before MA(s),After MA(s),Change Rate(%),MC Degranulation Ratio (%),N,8,9.190.65,-,-,10.62.6,M,8,5.610.48*,-,-,14.15.9,C,8,5.900.24*,5.590.52,-5.1,11.94.7,R,8,5.440.37*,7.520.65,38.5,75.74.4,L,8,6.130.82*,8.501.56,39.1,76.95.9,CR,8,5.270.62*,5.390.57,3.3,10.42.4,CL,8,6.050.43*,6.260.79,3.8,12.42.3,注：较正常组痛阈：*,P0.01,；较处理前痛阈：,P0.05,；较捻转组,(R),痛阈变化率：,P0.05,； 较捻转组,(L),痛阈变化率：,P0.05,； 较正常组或模型组,:, P0.05,；较捻转组或提插组,:, P0.05,。,2024/9/21,15,Analgesia effects observed in different groups,Group,n,Pain Threshold(PT),4d Before MA(s),After MA(s),Change Rate(%),MC Degranulation Ratio (%),N,8,9.190.65,-,-,10.62.6,M,8,5.610.48*,-,-,14.15.9,C,8,5.900.24*,5.590.52,-5.1,11.94.7,R,8,5.440.37*,7.520.65,38.5,75.74.4,L,8,6.130.82*,8.501.56,39.1,76.95.9,CR,8,5.270.62*,5.390.57,3.3,10.42.4,CL,8,6.050.43*,6.260.79,3.8,12.42.3,注：较正常组痛阈：*,P0.01,；较处理前痛阈：,P0.05,；较捻转组,(R),痛阈变化率：,P0.05,； 较捻转组,(L),痛阈变化率：,P0.05,； 较正常组或模型组,:, P0.05,；较捻转组或提插组,:, P0.05,。,2024/9/21,16,Analgesia effects observed in different groups,Group,n,Pain Threshold(PT),4d Before MA(s),After MA(s),Change Rate(%),MC Degranulation Ratio (%),N,8,9.190.65,-,-,10.62.6,M,8,5.610.48*,-,-,14.15.9,C,8,5.900.24*,5.590.52,-5.1,11.94.7,R,8,5.440.37*,7.520.65,38.5,75.74.4,L,8,6.130.82*,8.501.56,39.1,76.95.9,CR,8,5.270.62*,5.390.57,3.3,10.42.4,CL,8,6.050.43*,6.260.79,3.8,12.42.3,注：较正常组痛阈：*,P0.01,；较处理前痛阈：,P0.05,；较捻转组,(R),痛阈变化率：,P0.05,； 较捻转组,(L),痛阈变化率：,P0.05,； 较正常组或模型组,:, P0.05,；较捻转组或提插组,:, P0.05,。,collagen fibers of skin and muscle,Mast cells in skin,Group,Normal,Group,Inject,Control,collagen fibers of skin and muscle,Mast cells in skin,Connective Tissue Research,2008,，,50,（,2,）,: 110-120,Acupunc.,whirling,Acupunc.lift and thrust,collagen fibers of skin and muscle,Mast cells in skin,collagen fibers of skin and muscle,Mast cells in skin,Connective Tissue Research,2008,，,50,（,2,）,: 110-120,Mast cells degranulation before and after collagen destroyed,Collagen,destroyed,Collagen,destroyed,Connective Tissue Research,2008,，,50,（,2,）,: 110-120,collagen fibers and mast cells,in normal acupoint,collagen fibers enlacement and mast,cells degranulation while whirling,collagen fibers tightened and mast cells,degranulated while lifting and thrusting,collagen fibers were destroyed and,mast cells had no degranulation,Connective Tissue Research,2008,，,50,（,2,）,: 110-120,2024/9/21,21,The mast cells in acupoint may translate the mechanical signal into the biological information of neural system .,The collagen and mast cells degranulation are very important to the manual acupuncture analgesia effect.,Is this the same as electric acupuncture?,2024/9/21,22,组别,n,痛阈(s),痛阈变化率 (%),肥大细胞脱颗粒率(%),正常组,8,9.190.65,-,10.6,模型组,8,5.610.48*,-,14.1,手针组,8,7.520.65,38.5,75.4,电针组,8,8.671.06,45.9,73.3,胶原酶+手针组,8,5.390.57,3.2,10.4,胶原酶+电针组,8,8.301.23,47.6,19.0,色甘酸钠+手针组,8,6.000.48,5.8,25.1,色甘酸钠+电针组,8,7.340.70,27.5,29.5,手针及电针治疗前后,AA,大鼠痛阈和痛阈改变率以及穴区肥大细胞脱颗粒率的变化,注：与 正常组相比较：*,P0.05,；与 模型组相比较：,P0.05,； 与手针组相比较：, P0.05,；, P0.01,；与电针组相比较：,P0.05,。,3. 穴位皮下注射组胺的镇痛疗效,组别,n,痛阈(s),基础痛阈,造模后痛阈,治疗后痛阈,空白对照组,12,9.040.20,9.380.19,9.270.17,模型组,12,8.900.40,6.480.28,6.580.35,生理盐水对照组,12,9.200.28,6.090.30,6.610.31,手针组,12,9.520.18,6.580.17,8.770.26,组胺注射组,12,9.210.20,6.270.22,8.500.28,各组痛阈测量比较,3. 穴位皮下注射组胺的镇痛疗效,组别,n,痛阈(s),基础痛阈,造模后痛阈,治疗后痛阈,空白对照组,12,9.040.20,9.380.19,9.270.17,模型组,12,8.900.40,6.480.28,6.580.35,生理盐水对照组,12,9.200.28,6.090.30,6.610.31,手针组,12,9.520.18,6.580.17,8.770.26,组胺注射组,12,9.210.20,6.270.22,8.500.28,各组痛阈测量比较,针刺研究，,2010,，,35,（,2,）：,99-103,3. 穴位皮下注射组胺的镇痛疗效,组别,n,痛阈(s),基础痛阈,造模后痛阈,治疗后痛阈,空白对照组,12,9.040.20,9.380.19,9.270.17,模型组,12,8.900.40,6.480.28,6.580.35,生理盐水对照组,12,9.200.28,6.090.30,6.610.31,手针组,12,9.520.18,6.580.17,8.770.26,组胺注射组,12,9.210.20,6.270.22,8.500.28,各组痛阈测量比较,针刺研究，,2010,，,35,（,2,）：,99-103,3. 组胺H,1,受体在传递针刺信号中的作用,各组足三里穴位处脱颗粒比较,组别,N,脱颗粒率,空白组,12,33.59,0.72,模型组,12,39.71,2.09,生理盐水穴位注射组,12,36.67,2.36,手针针刺组,12,57.61,1.42,组胺穴位注射组,12,57.03,2.95,色甘酸钠预处理胺注射组,13,25.40,1.80,色甘酸钠预处理针刺组,12,36.03,2.28,组胺H1拮抗剂预处理针刺组,12,51.54,2.32,组胺H1拮抗剂组胺注射组,12,37.13,1.90,组胺H1拮抗剂穴位注射组,22,32.24,1.40,P0.01,与空白组比较（,vs. control,）；,P0.01,与模型组比较（,vs. model,）；,P0.01,与手针针刺组比较（,vs. control,）；,P0.01,与生理盐水穴位注射组比较；,P0.01,与组胺穴位注射组比较，,P0.01,与组胺,H1,拮抗剂穴位注射组比较。,针刺研究，,2010,，,35,（,2,）：,99-103,3. 组胺H,1,受体在传递针刺信号中的作用,各组足三里穴位处脱颗粒比较,组别,N,脱颗粒率,空白组,12,33.59,0.72,模型组,12,39.71,2.09,生理盐水穴位注射组,12,36.67,2.36,手针针刺组,12,57.61,1.42,组胺穴位注射组,12,57.03,2.95,色甘酸钠预处理胺注射组,13,25.40,1.80,色甘酸钠预处理针刺组,12,36.03,2.28,组胺H1拮抗剂预处理针刺组,12,51.54,2.32,组胺H1拮抗剂组胺注射组,12,37.13,1.90,组胺H1拮抗剂穴位注射组,22,32.24,1.40,P0.01,与空白组比较（,vs. control,）；,P0.01,与模型组比较（,vs. model,）；,P0.01,与手针针刺组比较（,vs. control,）；,P0.01,与生理盐水穴位注射组比较；,P0.01,与组胺穴位注射组比较，,P0.01,与组胺,H1,拮抗剂穴位注射组比较。,针刺研究，,2010,，,35,（,2,）：,99-103,穴位处组胺及H,1,受体,组胺参与到了针刺麻醉的穴位局部机制,通过H,1,受体进一步传递针刺信号,研究阐明了手针和电针穴位针刺有效信号的启动机制,MA,EA,Electric A.,Thank you,