【病毒外文文献】2009 Detection and Molecular Characterization of Gene 3 and 5 of Turkey Coronavirus from Turkeys with Severe Enteritis i

BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors nonprofit publishers academic institutions research libraries and research funders in the common goal of maximizing access to critical research Detection and Molecular Characterization of Gene 3 and 5 of Turkey Coronavirus from Turkeys with Severe Enteritis in Brazil Author s Amarilis N D elboux Bunger Jorge Luis Chac n Richard C Jones and Antonio J Piantino Ferreira Source Avian Diseases Digest 4 3 e5 e5 2009 Published By American Association of Avian Pathologists DOI http dx doi org 10 1637 8939 1 URL http www bioone org doi full 10 1637 8939 1 BioOne www bioone org is a nonprofit online aggregation of core research in the biological ecological and environmental sciences BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies associations museums institutions and presses Your use of this PDF the BioOne Web site and all posted and associated content indicates your acceptance of BioOne s Terms of Use available at www bioone org page terms of use Usage of BioOne content is strictly limited to personal educational and non commercial use Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder DETECTION AND MOLECULAR CHARACTERIZATION OF GENE 3 AND 5 OF TURKEY CORONAVIRUS FROM TURKEYS WITH SEVERE ENTERITIS IN BRAZIL AMARILIS N D ELBOUX BUNGER JORGE LUIS CHACO N RICHARD C JONES AND ANTONIO J PIANTINO FERREIRA Contact Address af piantino fmvz usp br Important Findings The presence of turkey coronavirus TCoV in 12 intestinal content samples from turkey flocks aged between 10 and 104 days with severe enteritis was monitored in the period of 2004 to 2006 Study results suggest that the strategy of amplification of the 39 untranslated region 39UTR followed by sequencing of genes 3 and 5 has proved to be an effective means of detection of TCoV in intestinal contents According to the genes sequenced the Brazilian TCoV seems to be different from those described previously Significance of Findings TCoV is a causative agent associated with poult enteritis and mortality syndrome PEMS in turkeys worldwide The disease is an acute highly contagious enteric disease of turkeys characterized by depression anorexia diarrhea and high mortality in commercial turkey flocks This is the first description of the molecular characteristics of TCoV in Brazil the data gathered are invaluable for the molecular epidemiology of coronavirus induced diseases in turkeys and may elucidate virus origin or recombinant events Further studies examining the genetic relationships between the S gene of TCoV and other avian coronaviruses are very important and necessary to establish the evolutionary relationships to improve coronavirus classification and to contribute to global epidemiology of TCoV Additional Information TCoV is one of the major etiologic agents of diarrhea in turkeys between 1 and 6 weeks of age and is characterized by anorexia severe diarrhea dehydration and weight loss TCoV is known as one of the enteropathogenic agents involved with PEMS in association with increasing mortality levels in turkey farms Besides the enteric effects this virus causes circulatory disorders that mediate bluecomb disease Some studies have demonstrated that TCoV shows a phylogenetic relationship with the infectious bronchitis virus and is therefore classified as a Group 3 coronavirus In Brazil little information has been published on the occurrence of PEMS in turkeys The first report of PEMS associated with turkey coronavirus and astrovirus which presented high mortality accentuated depression and low performance described outbreaks in 17 turkey flocks located in the south and southeast regions of the country Subsequently the presence of TCoV associated with PEMS in the southeast of Brazil has been described Copyright E 2009 American Association of Avian Pathologists Inc 1933 5334 online Summarized from Avian Diseases Vol 53 No 3 2009 Pages 356 362