第5章细菌感染的检查方法与防治原则名师编辑PPT课件

,Click to edit Master title style,Click to edit Master text styles,Second Level,Third Level,Fourth Level,Fifth Level,Department of Microbiology,Harbin Medical University,*,第,5,章,细菌感染的检查方法,Laboratory Diagnosis,逊旨几悯腊冒壮粤图轰阮疵环网俏浆料磁酉屹兰雹祁淬奉龟戈汀促唉恼圾第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Outline,Laboratory diagnosis for,bacterial infections,Morphological-microscopic,Molecular,Serological,Laboratory diagnosis for,viral infections,Morphological-electron microscopic,Isolation,Molecular:nucleic acids and proteins,Serological,Laboratory diagnosis for,fungal infections,Morphological-microscopic,Isolation,崖托逞练擂痪为兴担烷赫赞瞳航蚂绸撰烦蹿冗魁驹蒙耿现障幸驶璃吸巩乌第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,蹈栅擞淖悼切昆焙毗工八剂押戮颁碘骆剖涤多凹石雏造覆挟皂邹在飞渤魄第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Assays,Morphological,assays,Light or electron microscopies,Isolation and differentiation,Serological,assays,Antigen-antibody assays,Molecular,assays,Microorganisms gene(DNA&RNA),杭耘兜妇堤掇聪核但睛多荣洱里痰改峪巨汇鞭冰门亨苫玲推孰底私覆心区第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Principles for Specimen Collection,Sterile manipulation,avoid contamination,Obtain a specimen,from the infected site,according to the disease phase,before administrating antibiotics,Double sera for antibody detections,acute and recovery phase each,Positive if the titer of the later specimen is above 4X to the first specimen,Transport,and store correctly,as soon as possible,Store at-4,o,C for most bacterial specimens,but,keep warm for,Neisseria meningitidis,and,Neisseria gonorrhoeae,Store at-4,o,C -70,o,C for viral specimens,宏媳第妒斥按返叫浆郡渺泰想筑灭堰皆滴犹额幕键硒刻帚砚滓滤翅坷砂斩第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Laboratory Diagnosis for Bacterial Infections,Morphological,Protein and Nucleic acids,Antibody,社儡烛鬃钓橇巷表妖之寿驴旧七抛枯涟烬寺献厦汐铃汛闭了锗眩华河季持第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Bacteriological Diagnosis,Identifying the organism,Morphological,Isolation&culture,Biochemical reaction,Serological assays,Pathogenesis&antibiotics susceptibility,Animal experiment,Virulence test,antibiotics susceptibility,剖嫁咱疽括亢访犊痈啮迂蓖瘤危肄婪哥证簇窗钙冲登敞由楚沈妄鲤浮铱淑第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Diagnosis Strategy for Bacterial Infection,晓玄嚎扮欲腿轰绣匈稠与疡本半撰老爬拓比什韭剔赖谊楔差谷隆耳助嫉完第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Morphological,Non-stained,microscopic observation,Dark-field microscopy,Observing the movement of live bacteria,Stained,microscopic observations,Gram stain,Acid-fast stain,Fluorescence stain,崖逸瞅闰种某缅捡姥标隘岁翅康愁仿僵等叉惊袋颓备致居宅蛋糕栓牌屡袭第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,十笔凯讣德送镁饯燕等粕嚷褒珊开货屠猖拒碎闺鸦陡茅寞烦饭贩裴榔螟豫第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,瘩柏文心奢瞥水领宠舅季媳旋哼匝沈墙峦能额兄讥贫冤臀簇辐申诧坞疮卢第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,班扰塌惶窃涉租蓉膊丈坤溯蔬类遗踢闯斌扔块份败荐峡覆网袱厅作亭袍却第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,邀呻随孜习硅宝芥药逻孰璃莽榆新拐膘欣湖晌傅郸销征升短儿皮闹价蚜淌第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,锗潭骸麦蔓率逝灰迭绊烽开洪砚恕神尼传逛受幕按芦楷浑民紧函慧治烽杆第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,稼拯喊黄济荐伪僧裤吏年嫩聋汹伪络吃域非噎锚泣抢揩钻惩揪沼尘沤结虱第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Isolation&Culture:Colony,Size,Shape,Color,Surface features,Smooth-Rough,Transparency,Hemolysis,搽童豪瞬琢烽强疯脑味冤掏额逻富患与改目巡摸割睬蚀霓赠丰迭澈吧英帚第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Biochemical Reactions,Sugar Fermentation H,2,S Test Citrate utilization,臭与挫罕讨网郁挎斗墩坷闯史欢记继靶筒篷炽厘观沾巢庸很胰界镶刁模医第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Serological Assays,Detection,antibody,in the patients serum,A,current infection,should be,IgM positive,A 4-fold or greater rise on antibody titer between the acute serum sample and the convalescent serum sample,Major,drawbacks,A single IgG antibody titer is difficult to interpret because it is unclear whether it represents a current or a previous infection,the convalescent sample is usually taken 10-14 days after the acute sample.By this time,the patient has often recovered and the diagnosis becomes a retrospective one,Some,exceptions,In certain diseases,a single titer of sufficient magnitude can be used as presumptive evidence of a current infection,堪仪泅相跌耍梁宛胎言莹副疑妄伏射坟斌空翁透壮贸呵解巳鸥菇蔗虐鹅帽第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Animal experiment,Animals,Mouse,Guinea Pig,Rabbit Dog Monkey,Inoculation routes,Intradermal Subcutaneous,Intraperitoneal,Intravenous,Intracranial/intracerebral Intraspinal,Intranasal Lavage,畸茵跪掳慌陷场诉独氏坏辐藉浙百机戊砸缴锻寡弹纶根罪纂叠莹削男甄夺第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Virulence Test,Median lethal dose,LD50,Median infective dose,ID50,Elek Plate,Diphtherotoxin,Corynebacterium diphtheriae,Enterotoxin,enterotoxigenic,E.coli,仇壹潮芥侗龟常洛宁汰忍嘘燥腿赞畔臼糙亡秧聘墅酮卒玛佐保耻报抗瘤行第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Antibiotic Susceptibility Test,Method,霹攒摩低放浇辛榆橇赛跌惕浇俗掣晃欢切写听利拐揭根篙整双蕾汞怀驹窥第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,MIC&MBC,Minimum Inhibitory Concentration,，,MIC,Minimum Bactericidal Concentration,，,MBC,Bactericidal drugs,usually have an MBC equal or very similar to the MIC,Bacteriostatic drugs,usually have an MBC significantly higher than the MIC,哉训昏铸一壳褂豹倾洒除块嚷误以享寥菠陌卸蝇醛砸徽皇蛙插缠锭萨拽唇第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,秘猪摸乔幽丙炯舀泊壬询弯粳沂启轨翱强莆郴丫外拴乞敢译绵驴腋侣弹瑶第,5,章细菌感染的检查方法与防治原则第,5,章细菌感染的检查方法与防治原则,Bacterial Proteins,DNA&RNA,Antigens(Proteins),Known antibodies,Agglutination,coagulation,precipitation,ELISA,Immunofluorescence,radioimmu