犬猫寄生虫病粪便检查法

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,*,*,Experiment III,Examination of Fecal samples,翁亚彪 朱建荣 宋慧群 袁子国 李娟 丘丽玲,华南农业大学兽医学院,犬猫寄生虫病粪便检查法,1.Place a drop of isotonic saline on a slide.,2.Take a small piece of feces with a toothpick,and stir it into the saline.,3.Add a coverslip or not,and examine,microscopically.,一、直接涂片法Direct Smear,二、饱和盐水浮卵法Fecal Flotation,Mix several grams(a teaspoonful)of feces with the flotation solution in a plastic cup.,Strain the mixture through a cuprum strainer(60 mesh)into another plastic cup.,Allow the flotation to stand for at least 5 minutes.,Take a few drops of the surface of the solution with cuprum wire loop on a microscope slide,examine them under the mecroscope with the 10,lens.,三、沉淀法Fecal Sedimentation,Mix about 100ml of water with about 10g of feces and place in a plastic cup.,Strain the mixture through a cuprum strainer(60 mesh)into another cup.,Allow mixture to sit for 10 minutes and then decant the supernatant.,Add more water,mix,and repeat the sedimentation procedure until the supernatant will be very clear.,Stir remaining mixture and place a few drops on a microscope slide,examine them under the mecroscope with the 10 lens.,华枝睾吸虫卵,球虫卵囊,粪检中镜下常见杂质,1.植物导管：梯纹、网纹、,孔纹,2.螺纹和环纹,3.管胞,4.植物纤维,5.小麦的颖毛,6.真菌的孢子,7.谷壳的一些局部,8.稻米胚乳,9.植物的薄皮细胞,10.植物的薄皮细胞,11.淀粉粒,12.花粉粒,13.植物线虫的一些虫卵,14.螨的卵未发育,15.螨的卵已发育,11,实验报告,常用粪检法有哪几种，各有何优缺点？,直接涂片法,饱和盐水浮卵法,沉淀法,