免疫荧光分型指南共41张课件

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,*,免疫荧光分型指南,丁桃,加拿大公共卫生署,国家HV免疫实验室,昆明20193,OUREI,概要,样品处理,单克隆抗体,组,滴定,补偿,荧色物,绝对数校准荧光球,通用分析模板,OUREI,样品处理,溶解免洗的方法,单克隆50-100全血,溶解液,抗体,孵化10/15分钟,孵化10/15分钟,流式细胞仪分析,OUREI,建议,加入2%的多聚甲醛固定,绝对记数时荧光球和血液移液的准确性,加过荧光球的样品,必须在当天上机分析,在上机采集数据时,应在达到至少1微升血样之,后才能停止;即如果荧光球的浓度为每微升,1000,应收集到至少1000个荧光球才能停止采集,OUREI,一抗体组合,OUREI,不同的抗体组合,双色:,Facscount and Guava:CD3/CD4,PLG:CD45/4,三色:,BD and BC:CD4/3/45.CD4/8/3,四色:,BD and BC:CD4/8/3/45,OUREI,应使用哪种抗体组合?,CD4/CDS/CD3,CD16/CD19/CD3,CD45/CD4,CD45/CDS,CD45/CD3/CD4,CD45/CD3/CD8,CD4,NIH AND CDC指南,Cytometry IClinical Cytometry,Original Articles,Use of CD45 Gating in Three and Four-Color Flow,Cytometric Immunophenotyping:Guideline From The,National Institute of Allergy and Infectious Diseases,Division of aids,Carol T.Schnizlein-Bick,Francis F.Mandy,Maurice R.G.OGorman,Helene Paxton.,Janet K-A.Nicholson,Lance E Hultin,Rebecca S Gelman,Cynthia L wilkening,and Daniella Livnar,VoL.52/RR-2,Recommendations and Reports,Guidelines for Performing Single-Platform Absolute CD4*T-Cell,Determinations with CD45 Gating for Persons Infected,with Human Immunodeficiency virus,Francis F.Mandy,PhD,Ja stk a nelson m,OUREI,cD45的使用,CD45可标记所有的白细胞,CD45对淋巴细胞的表达大大高于单核细,胞和巨噬细胞,故用来识别淋巴细胞,OUREI,cD45异相门控:一个稳固的方法,样品年龄,cD45/ss异相门,FS/SS均相门,天,CD4 FIT(,4天,Schnizlein-Bick et al.2019,OUREI,