小单孢菌属和拟诺卡氏菌属.ppt

稀有放线菌的分离与培养,报告人：张晓敏,报 告 内 容,分离培养基的选择 小单孢菌形态与生理生化特征 拟诺卡氏菌形态与生理生化特征 小单孢菌、拟诺卡氏菌与链霉菌区别,分离培养基的选择,淀粉-酪蛋白硝酸盐琼脂培养基 溶胶几丁质琼脂培养基 甘露醇-利福平琼脂培养基,淀粉-酪蛋白硝酸盐琼脂培养基,starch-casein nitrate agar ： starch 10g ，sodium caseinate 0.3g，KNO3 2g，agar 15g， PH 7.0-7.4. 37 g novobiocin，223g mycostatin 30 培养 21天 Micromonospora chaiyaphumensis sp. nov Micromonospora eburnea sp. nov,基础培养基：K2HPO4 0.7g，KH2PO4 0.3 g，MgSO47H2O 0.5g，FeSO47H2O 0.01g，ZnSO4 0.001g，琼脂10 克，蒸馏水1.0L.PH7.0 121 蒸汽灭菌20 min,每100 mL加20 mL胶体几丁质悬浮液和5 mg放线菌酮,溶胶几丁质琼脂培养基,甘露醇-利福平琼脂培养基,mannitol-rifampicin agar ： 甘露醇 10g，酵母提取物 7g，酪蛋白水解物 2g，蛋白胨 1g， NaCl 1 g， CaCO3 0.2 g，制霉菌素 100mg,利福平 5 mg 27 培养6周 Nocardia acidivoran,返回,Micromonospora,gyrB基因序列分析 和 DNADNA 杂交 15 species: Micromonospora aurantiaca, Micromonospora carbonacea, Micromonospora chalcea, M. chersina, Micromonospora coerulea, Micromonospora echinospora, Micromonospora gallica, Micromonospora halophytica, Micromonospora inositola, M. matsumotoense, Micromonospora nigra, M. olivasterospora, Micromonospora pallida, Micromonospora purpureochromogenes and M. rosaria.,小单孢菌形态与生理生化特征,Aerobic, Gram-positive, mesophilic, non-motile actinomycete well-developed and branched substrate hyphae. Aerial mycelium was absent. Spore chains were absent. Mycolic acids were absent. Acyl type of cell wall muramic acid is glycolyl. Fatty-acid type:3b. The predominant menaquinone: MK-9（H4）、MK-9（H4H6H8） Characteristic whole-cell sugars are xylose and arabinose. Phospholipids :P-II型 . Cell-wall peptidoglycan :CW-II (glutamic acid,alanine and meso-diaminopimelic acid). DNA G+C(mol%): 71%-73% .,well-developed and branched substrate hyphae. Aerial mycelium was absent. At maturity, spores were spherical to oval, appeared to be rough and were non- motile (Fig. 1). colour of the vegetative mycelium on ISP 2 medium is orange, turning to gre yish black or black upon sporulation.,growth at pH4-8 and25-30,no growth above 45 Brown, diffusible pigment positive for the utilization of L-arabinose, D-fructose, D-galactose, D-glucose,lactose, melibiose, salicin and D-xylose. negative for nitrate reduction and the utilization of D-mannitol and rhamnose.,Micromonospora chaiyaphumensis,Single, non-motile, spherical spores (0.60.9 mm) were formed on substrate hyphae. Aerial mycelium is absent. The spore surface appears warty. Spores are non-motile.,The colour of the spulating colonies was yellowish white to pale orange. A yellow, soluble pigment Utilizes L-arabinose, D-fructose, D-galactose, D-glucose, lactose, D-ribose, salicin and D-xylose; weak utilization of cellobiose, not utilize glycerol, D-mannitol, melibiose, raffinose or L-rhamnose.,Micromonospora pattaloongensis,well-developed and branched substrate hyphae. Single, non-motile spores are formed on substrate hyphae ，0.45 mm in diameter. Aerial mycelium is absent. The spore- surface appears rough and nodular. Colonies are yellowish white and turn greyish black after sporulation Pale yellow soluble pigment positive for nitrate reduction, Utilizes D-glucose, L-rhamnose, D- melibiose, D-rafnose,glycerol, salicin, lactose, cellobiose and D-xylose; weakly utilizes D-mannitol and D-galactose but not L-arabinose,D-fructose or D-ribose.,Micromonospora eburnea,生理生化区别,返回,拟诺卡氏菌属,原拟诺卡氏菌属包括 7个种 2个亚种 . Gr u n d等 根据化学分类和数值分类研究结果 将 原 拟 诺 卡 氏菌 属 中 的 MK一 9 、PII或 PIV型磷 酸 类 脂 的 5个 种 转 入糖 丝 菌 属。 原达松维尔拟诺卡氏菌的 2个亚种分别命名为达松维尔拟诺卡氏菌、 白色拟诺卡氏菌白色亚种和白色拟诺卡氏苗葱绿亚种 耐碱苗白浅红链霉菌转入该属而命名为白浅红拟诺卡 氏菌,拟诺卡氏菌属的鉴别特征,拟诺卡氏菌属的生理生化特征,返回,谢 谢,