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1 2 3 hplc 80% 1 hplc 2 3 hplc3m5m7m10m1m10mhplc50000/m160000/m100-300mm 4 hplc 5 11 1. 2 2 1 2 35 1 ph 2 3 4 55 6 7 :1 2 : :1. (lc-10at)phenomenexo 10l 2 2.0l/ml2.0l/ml1.0l/ml2.0l/ml5.0l/ml10.0l/ml 12.1ml/min30354nm 310l10l 10l2.0l/ml10l2.0l/ml(35) 410l1.0l/ml2.0l/ml5.0l/ml 10.0l/ml10l 5 12.001.7570%1.501.251.000.750.500.250.00min=60%40%70%30%80%20%=80%20% 25.04.54.03.53.02.52.01.51.00.50.0-0.5min 31 ul/ml, 2 ul/ml, 5 ul/ml, 10 ul/ml412- 31 23 44123 5. (1)(2)(3)45(4)(5)510lm 201440150161. 2. (hplc)hplc11.(1.51074.5107pa)(0.1mlmin-1)3. 4. 530cm45mm()5. 6. hplc(1) (2) 10-20(3) hplc(4) hplcmanual(5) manualpurgestart10mlmin-1(6) 2000injureon(7) fileselect?users?and?methodsnew?methodprotocola.2-5b.c.loading-injectd.(8) startpostrun(9) (10) 1. millipore0.2m0.45m-5ml/min50ml4mol/l2. 10303. (1:4,v/v)15 1010()4. 20ml10ml30
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