各种激发光和吸收光波长

各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。这对CONFOCAL去做多重染邑时是必 不可少的参考资料。全部文件请到这里下载！Exci tat ion and Emission Wave I engths of FluorophoresTavis HFIuoroTableH: Common fIuorophores(from Zeiss Corporation website)FluorophoreAbsorptio Emission Other infon1,5 IAEDANS3364901,8-ANS3724804-Methy I umbel Ii ferone3855025-carboxy-2,7-d ich IorofIuoresce in5045295-CarboxyfIuorescei n (5-FAM)4925185-CarboxynapthofIuoresce i n (pH 10)512/598563/668 Ratio Dye, pH5-Carboxytetramethy Irhodami ne542568(5-TAMRA)5-FAM (5-CarboxyfIuorescei n)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodami ne)5786045675915-TAMRA548552(5-Carboxytetramethy Irhodami ne)5425686-Carboxyrhodami ne 6G5185436-CR 6G5185436-JOE5205487-Ami no-4-methy1coumar i n3514307-Ami noact i nomyc i n D (7-AAD)5466477-Hydroxy-4一methy 1coumar i n360449, 4559-Am i no-6-ch1oro-2-methoxyacr i d i ne412, 430471, 474ABQ344445Ac i d Fuchs i n540630ACMA412, 430471, 474(9-Ami no-6-ch1oro-2-methoxyacr i d i ne)Acr id ine Orange + DNA502526Acr id ine Orange + RNA460650Acr idine Orange, both DNA & RNA440-480520-650Acr id ine Red455-600560-680Acr id ine Yellow470550Acr if lav in436520Acr if lavi n Feulgen SITSA355-425460Aequor i n (Photoprote i n)466AFPs 一 AutoFluorescent Protein -(Quantum Biotechnologies) see sgGFP,sgBFPAlexa Fluor 350346442342441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa Fluor 546556, 557572, 573Photoprotei nAlexa Fluor 568577,578603Alexa Fluor 594590,594617, 618Alexa Fluor 633632650Alexa Fluor 647647666Alexa Fluor 660668698Alexa Fluor 680679702Al i zar in Comp lexon530-560,580,580624-645Al izarin Red530-560580Al 1ophycocyanin (APC)630,645655, 660AMC, AMCA-S345445AMCA (Ami nomethy1coumar i n)345425347444AMCA-X353442Ami noact i nomyc i n D555655Aminocoumar in346442350445Ami nomethy1coumar i n (AMCA)345425347444Ani 1in Blue600Anthrocy1 stearate360-381446APC (A 11ophycocyani n)630,645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530 Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabr i ne436490ATTO-TAG? CBQCA465560ATTO-TAG? FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisaminophenyloxadi azole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)381, 382,445, 447Blue Fluorescent Protein383448BFP/GFP FRETBi mane398490Bisbenzamide360461Bi sbenz imide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Blancophor FFG390470Blancophor SV370435BOBO7-1462481BOBO7-3570602Blue Fluorescent ProteinRatio Dye, Zn2+ue shifted GFP (Y66H)Bod i py 492/515490515Bod i py 493/503533549Bod i py 500/510509515Bod i py 505/515502510Bod i py 530/550528547Bod i py 542/563543563Bod i py 558/568558569Bod i py 564/570564570Bod i py 576/589579590Bod i py 581/591584592Bod i py 630/650-X625642Bod i py 650/665-X647665Bod i py 665/676605676Bodipy Fl504,505 511, 513Bod i py FL ATP505514Bodipy Fl-Ceramide505511Bod i py R6G SE528547Bod i py TMR542574Bod i py TMR-X con jugate544573Bod i py TMR-X, SE544570Bod i py TR589617Bod i py TR ATP591620Bod i py TR-X SE588616B0-PR07-1462481B0-PR07-3544570Br i 1 1i ant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - at io Dye, Zn2+459/417517/532Ratio Dye, Zn2+Ca1ce i n494517Ca1cein Blue373440Calcium Cr imson58&589611,615Calcium Green501,506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium Orange549575576CaI cofluor White385,395, 437,440,405445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue377420398423399Cascade Yellow399550400552Catecholami ne410470CCF2 (GeneBlazer)CFDA494520CFP 一 Cyan Fluorescent Protein430,433, 474,475,Cyan Fluorescent Protein436,(453)476,(501)CFP/YFP FRETCh IorophyI I480650Chromomyc i n A436-460470Chromomyc i n A445575CL-NERF (Rat i oDye,pH)504/514540Ratio Dye,pHCMFDA494520Coe IenterazineCa2+Dye, (429)465Ca2+ Dye, bio Iuminescence,b i oIumi nescencenat i ve mo IeculeCoe Ienterazinecp (Ca2+ Dye,)(430)442Ca2+ Dye,biolumi nesceneeCoe Ienterazinef(437)473Ca2+ Dye,bio IuminescenceCoe Ienterazinefcp452Ca2+ Dye,bio IuminescenceCoe Ienterazineh(437)464Ca2+ Dye,bio IuminescenceCoe Ienterazinehep(433)444Ca2+ Dye,biolumi nesceneeCoe Ienterazineip441Ca2+ Dye,bio IuminescenceCoe Ienterazinen(431)467Ca2+ Dye,bio luminesceneeCoe Ienterazine0460575Coumarin PhaIloidin387470C-phycocyanineCPM MethyIcoumar i n384469MethyIcoumar i nCTC400-450602CTC FormazanCy2489506Cy3. 1 8554568Cy3.5581598Cy3514566552570554Cy5. 1 8649666Cy5.5675695Cy5649666670Cy7710,743767,805Cyan GFP433(453)475(501)eye Ii c AMP FIuorosensor (F i CRhR)500517CyQuant Cel I Proliferation Assay480520DabcyI453DansyI340578DansyI Amine337517DansyI Cadaverine335518DansyI Ch lor i de372518Dansyl DHPE336517DansyI fluor ide356noneDAP I359461DapoxyI403580DapoxyI 2374574DapoxyI 3373574DCFDA504529DCFH(Diehlorod i hydrofIuoresce i n505535Di acetate)DDAO463607I Proliferation AssayDHR (D i hydorhodam i ne 123)505534Di-4-ANEPPS496705D i-8-ANEPPS (non-ratio)488605498713DiA (4-Di-16-ASP)456591Diehlorod ihydrofIuoresce in505535Di acetate (DCFH)DiD - Lipophi Iic Tracer644665Lipophi Iic TracerDiD (Di 1018(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535Dil (Di 1018(3)549,551565Di nitrophenol349DiO (DiOC18(3)484,487501, 502DiR748780Lipophi Iic TracerDiR (Di 1018(7)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447Enhanced Blue FluorescentProte i nECFP436474Enhanced Cyan FluorescentProteinEGFP48& 498507, 516Enhanced Green FluorescentProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthDT)528617Euchrysin430540EukoL ightEuropium (III) chlorideEYFP513, 520527, 532EnhancedYe I IowFluorescent ProteinFast Blue360440FDA494520Feu I gen (Pararosan i Ii ne)570625FIF(Forma IdehydInduced405433Fluorescence)FITC490, 494520, 525FITC Antibody493517Fl azo Orange375-530612FIuo-3480 ?506,520, 527506F1uo-4494516Fluorescein (FITC)490,494 520,525Fluorescein Di acetate494520Fluoro-Emerald495524F1uoro-Go1d (Hydroxyst i1 bami d i ne) 361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red?(high pH)572657Fura Red?Fluo-3Fura-2, high calcium335505Exci tat ionratio dyeFura-2, low calcium363512Exci tat ionratio dyeFura-2/BCECFGenacryI Br i I Ii ant Red B520590GenacryI Brilliant Ye I Iow1OGF430485GenacryI Pink 3G470583GenacryI Ye I low 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UVexcitation475509(wtGFP)GFP wiId type, UV excitation (wtGFP)395509GFPuv385508G1 oxa 1ic Acid405460Granular Blue355425Haematoporphyr in530-560580Hoechst 33258345487Hoechst 33342347Fi Iter sets insta11ed on the Axiovert 200M:1)Br ightfieldDIC:filter cube contains DIC ana Iyzer2) DAP I /BFP:Chroma set 31041, Ex at 380 nm (bandwidth 30 nm), Em at 460 nm(bandwidth 50 nm).3) FITC/GFP:Chroma set 41001, Ex at 480 nm (bandwidth 40 nm), Em at 535 nm (band width 50 nm)4) RHOD：Chroma set 41002c, Ex at 545 nm (bandwidth 30 nm), Em at 620 nm (bandv/idth 60 nm)5) Cy5:Chroma set 41024, Ex at 620 nm (bandwidth 60 nm), Em 665 nm and above (long pass filter).Also avai I able upon request:4) GFP：Chroma set 41017, Ex at 470 nm (bandwidth 40 nm), Em 525 nm (band width 50 nm)5) CFP:Chroma set 31044v2, Ex at 436 nm (bandwidth 20 nm), Em 480 nm(bandwidth 40 nm)6) YFP：Chroma set 4102& Ex at 500 (bandwidth 20 nm), Em 535 (bandwidth30 nm)7)CFP/YFPChroma set 31052, Ex 436 (bandwidth 20 nm), Em 535 (bandwidthFRET:30 nm)