左娜娜创新答辩ppt-阿卡宁衍生物合成产物中乙酰胆碱酯酶

前导物 合成 分离提纯测定生物活性 化学预试 传统方法的局限性：1)只适合易分离体系；2)只注重主要合成产物的信息NNH2OHOHOOOOOHOH OOOOOHOH OOHOHOHOOOONHNNHNNHNOOOOHOHNNOOHOHOHNNOOHOHNNOOOO HO HNSOO HOHOHNSOOONS399051015200.00150.00200.00250.00300.0035OD432抑 制 剂 用 量/g051015200.00100.00150.00200.00250.00300.0035OD432抑 制 剂 用 量/g图三图三 混合物经混合物经TLC的分离结果的分离结果用薄层色谱法可以鉴别出具有抑制能力的化合物。用薄层色谱法可以鉴别出具有抑制能力的化合物。min0246810121416mAU-8-6-4-20 DAD1 B,Sig=500,8 Ref=600,100(ZNN05051905051902.D)2.045 2.716 5.907 13.102 14.044 15.057 15.658min051015202530mAU-8-6-4-202 DAD1 B,Sig=500,8 Ref=600,100(ZNN05051905051905.D)2.147 2.304 12.648 13.251 19.701 20.131 20.505 20.935R T:0.0 0 -2 1.1 101234567891 01 11 21 31 41 51 61 71 81 92 02 1T im e (m in)02 0 0 0 0 04 0 0 0 0 06 0 0 0 0 08 0 0 0 0 01 0 0 0 0 0 01 2 0 0 0 0 01 4 0 0 0 0 01 6 0 0 0 0 01 8 0 0 0 0 02 0 0 0 0 0 02 2 0 0 0 0 02 4 0 0 0 0 02 6 0 0 0 0 02 8 0 0 0 0 03 0 0 0 0 0 03 2 0 0 0 0 03 4 0 0 0 0 03 6 0 0 0 0 0uAU1 2.1 64.2 92.4 51 1.6 61 0.0 62.7 73.1 99.3 51 0.2 75.3 42.2 58.2 45.4 81 2.8 87.8 51 3.7 01 4.7 31.8 41 7.8 01 5.4 81 8.8 11 9.8 7N L:3.7 0 E 6C h a n n e l A U V p yrid in ep y r i d i n e#3 2 7R T:1 2.4 7A V:1S B:71 1.7 9-1 2.2 5N L:5.5 4 E 5T:-c E S I F u l l m s 1 5 0.0 0-1 5 0 0.0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0m/z05 0 0 0 01 0 0 0 0 01 5 0 0 0 02 0 0 0 0 02 5 0 0 0 03 0 0 0 0 03 5 0 0 0 04 0 0 0 0 04 5 0 0 0 05 0 0 0 0 05 5 0 0 0 0Intensity4 6 3.24 6 4.33 6 1.24 8 5.18 4 8.38 2 3.42 6 9.23 6 2.35 6 7.15 8 7.06 5 7.17 3 5.97 9 2.68 8 0.37 1 2.15 4 3.05 1 8.33 5 6.74 1 3.23 7 4.92 8 9.46 2 9.86 8 5.51 9 7.32 6 8.34 4 3.21 7 6.7OOOOOHOHNHNp y r i d i n e#3 1 1R T:1 1.8 6A V:1N L:3.0 7 E 5T:-c E S I F u l l ms 1 5 0.0 0-1 5 0 0.0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th (n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 02 6 0 0 0 02 8 0 0 0 03 0 0 0 0 0Intensity4 6 1.2 33 6 1.2 74 6 2.2 43 6 2.2 43 9 3.2 38 7 8.2 98 7 6.9 76 4 0.5 96 7 7.0 54 8 3.0 33 4 1.0 35 5 0.9 28 9 8.6 65 9 1.0 68 1 6.0 25 0 0.4 07 6 8.1 93 0 9.3 67 0 1.7 65 7 8.7 24 4 0.4 52 4 9.2 01 7 4.8 93 0 6.9 72 2 4.2 6OOOOOONHNOOOOOOHNNp yrid in e#2 7 1R T:1 0.3 2AV:1N L:1.0 1 E 6T:-c E S I F u ll m s 1 5 0.0 0-1 5 0 0.0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 0w a ve le n g th (n m)05 0 0 0 01 0 0 0 0 01 5 0 0 0 02 0 0 0 0 02 5 0 0 0 03 0 0 0 0 03 5 0 0 0 04 0 0 0 0 04 5 0 0 0 05 0 0 0 0 05 5 0 0 0 06 0 0 0 0 06 5 0 0 0 07 0 0 0 0 07 5 0 0 0 08 0 0 0 0 08 5 0 0 0 09 0 0 0 0 09 5 0 0 0 01 0 0 0 0 0 0Intensity3 9 3.2 53 0 9.2 23 9 4.2 84 7 7.1 46 5 5.4 64 1 5.3 12 8 1.2 65 1 7.0 43 6 3.0 57 5 5.1 16 4 2.5 15 8 4.4 86 7 0.7 67 2 3.0 43 1 0.3 75 5 3.2 54 7 5.0 06 1 0.5 72 7 9.3 52 2 3.1 91 9 1.0 1OOHOHOHNNOOHOHHNONp y r i d i n e#2 4 9R T:9.4 8A V:1N L:3.3 5 E 5T:-c E S I F u l l ms 1 5 0.0 0-1 5 0 0.0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th (n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 02 6 0 0 0 02 8 0 0 0 03 0 0 0 0 03 2 0 0 0 0Intensity3 9 3.2 08 0 9.2 08 1 0.1 54 1 5.1 53 7 8.2 57 6 5.8 48 3 3.4 56 8 9.3 93 6 2.3 06 6 8.1 53 0 9.1 78 8 1.5 75 3 1.1 57 6 8.5 14 9 4.7 64 2 3.1 96 0 6.8 77 2 4.9 38 0 8.3 25 6 2.6 33 3 1.0 12 7 2.1 82 3 3.1 41 8 9.0 2p y r i d i n e#1 1 3R T:4.2 8A V:1N L:4.0 2 E 5T:-c E S I F u l l ms 1 5 0.0 0-1 5 0 0.0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th (n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 02 6 0 0 0 02 8 0 0 0 03 0 0 0 0 03 2 0 0 0 03 4 0 0 0 03 6 0 0 0 03 8 0 0 0 04 0 0 0 0 0Intensity4 0 9.1 84 4 3.2 65 4 7.1 83 9 1.0 95 2 5.1 13 3 9.2 47 7 9.3 34 5 3.2 77 3 7.1 35 1 3.1 37 1 8.5 33 5 2.9 48 1 9.3 32 5 3.2 95 6 6.7 06 3 9.0 55 0 8.9 83 1 1.1 86 5 4.3 28 4 3.1 28 8 4.5 62 0 2.1 22 3 0.1 2p y r i d i n e#3 1 7R T:1 2.0 9A V:1N L:2.6 0 E 5T:-c E S I F u l l ms 1 5 0.0 0-1 5 0 0.0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th (n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 0Intensity4 6 1.2 03 6 1.2 54 8 3.0 54 0 5.3 27 7 7.0 94 0 6.3 95 8 3.0 95 6 5.1 47 4 5.8 77 0 6.4 28 2 2.7 13 9 3.2 64 9 6.5 96 4 3.0 15 8 4.2 14 3 8.6 25 6 2.4 68 6 9.9 63 3 3.1 66 9 7.7 77 3 0.6 97 8 8.2 75 4 2.8 73 2 2.8 62 7 1.1 11 8 3.2 42 4 9.0 5p y r i d i n e#3 2 3R T:1 2.3 2A V:1N L:2.8 1 E 5T:-c E S I F u l l ms 1 5 0.0 0-1 5 0 0.0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th (n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 02 6 0 0 0 02 8 0 0 0 0Intensity3 6 1.2 14 6 3.1 64 6 1.1 18 7 7.1 84 6 4.2 38 4 4.9 16 9 2.8 34 9 7.0 63 6 3.2 94 0 5.6 35 4 5.0 28 0 9.2 86 6 2.9 85 8 9.5 17 4 4.8 93 8 3.0 68 7 9.2 03 4 3.3 24 2 2.5 96 4 1.0 95 0 6.7 67 1 0.7 03 2 3.2 68 9 5.5 52 7 2.9 91 8 3.3 62 4 1.0 6 感谢导师马志玲副教授的悉心指导 感谢吴京洪、凌连生 等老师的关心 感谢师兄师姐对我的鼓励和热心帮助感谢我的同学对我的照顾和支持谢谢观看/欢迎下载BY FAITH I MEAN A VISION OF GOOD ONE CHERISHES AND THE ENTHUSIASM THAT PUSHES ONE TO SEEK ITS FULFILLMENT REGARDLESS OF OBSTACLES.BY FAITH I BY FAITH