左娜娜创新答辩ppt-阿卡宁衍生物合成产物中乙酰胆碱酯酶

前导物 合成 分离提纯测定生物活性 化学预试 传统方法的局限性： 1)只适合易分离体系； 2)只注重主要合成产物的信息NNH2OHOHOOOOOHOH OOOOOHOH OOHOHOHOOOONHNNHNNHNOOOOHOHNNOOHOHOHNNOOHOHNNOOOO HO HNSOO HOHOHNSOOONS399051015200.00150.00200.00250.00300.0035OD432抑 制 剂 用 量 /g051015200.00100.00150.00200.00250.00300.0035OD432抑 制 剂 用 量 /g图三图三 混合物经混合物经TLC的分离结果的分离结果用薄层色谱法可以鉴别出具有抑制能力的化合物。用薄层色谱法可以鉴别出具有抑制能力的化合物。min0246810121416mAU-8-6-4-20 DAD1 B, Sig=500,8 Ref=600,100 (ZNN05051905051902.D) 2.045 2.716 5.907 13.102 14.044 15.057 15.658min051015202530mAU-8-6-4-202 DAD1 B, Sig=500,8 Ref=600,100 (ZNN05051905051905.D) 2.147 2.304 12.648 13.251 19.701 20.131 20.505 20.935R T : 0 .0 0 - 2 1 .1 101234567891 01 11 21 31 41 51 61 71 81 92 02 1T im e (m in )02 0 0 0 0 04 0 0 0 0 06 0 0 0 0 08 0 0 0 0 01 0 0 0 0 0 01 2 0 0 0 0 01 4 0 0 0 0 01 6 0 0 0 0 01 8 0 0 0 0 02 0 0 0 0 0 02 2 0 0 0 0 02 4 0 0 0 0 02 6 0 0 0 0 02 8 0 0 0 0 03 0 0 0 0 0 03 2 0 0 0 0 03 4 0 0 0 0 03 6 0 0 0 0 0uAU1 2 .1 64 .2 92 .4 51 1 .6 61 0 .0 62 .7 73 .1 99 .3 51 0 .2 75 .3 42 .2 58 .2 45 .4 81 2 .8 87 .8 51 3 .7 01 4 .7 31 .8 41 7 .8 01 5 .4 81 8 .8 11 9 .8 7N L :3 .7 0 E 6C h a n n e l A U V p yrid in ep y r i d i n e # 3 2 7R T : 1 2 .4 7A V : 1S B : 71 1 .7 9 - 1 2 .2 5N L : 5 .5 4 E 5T : - c E S I F u l l m s 1 5 0 .0 0 - 1 5 0 0 .0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0m /z05 0 0 0 01 0 0 0 0 01 5 0 0 0 02 0 0 0 0 02 5 0 0 0 03 0 0 0 0 03 5 0 0 0 04 0 0 0 0 04 5 0 0 0 05 0 0 0 0 05 5 0 0 0 0Intensity4 6 3 .24 6 4 .33 6 1 .24 8 5 .18 4 8 .38 2 3 .42 6 9 .23 6 2 .35 6 7 .15 8 7 .06 5 7 .17 3 5 .97 9 2 .68 8 0 .37 1 2 .15 4 3 .05 1 8 .33 5 6 .74 1 3 .23 7 4 .92 8 9 .46 2 9 .86 8 5 .51 9 7 .32 6 8 .34 4 3 .21 7 6 .7OOOOOHOHNHNp y r i d i n e # 3 1 1R T : 1 1 .8 6A V : 1N L : 3 .0 7 E 5T : - c E S I F u l l ms 1 5 0 .0 0 - 1 5 0 0 .0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th ( n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 02 6 0 0 0 02 8 0 0 0 03 0 0 0 0 0Intensity4 6 1 .2 33 6 1 .2 74 6 2 .2 43 6 2 .2 43 9 3 .2 38 7 8 .2 98 7 6 .9 76 4 0 .5 96 7 7 .0 54 8 3 .0 33 4 1 .0 35 5 0 .9 28 9 8 .6 65 9 1 .0 68 1 6 .0 25 0 0 .4 07 6 8 .1 93 0 9 .3 67 0 1 .7 65 7 8 .7 24 4 0 .4 52 4 9 .2 01 7 4 .8 93 0 6 .9 72 2 4 .2 6OOOOOONHNOOOOOOHNNp yrid in e # 2 7 1R T : 1 0 .3 2AV: 1N L : 1 .0 1 E 6T : - c E S I F u ll m s 1 5 0 .0 0 -1 5 0 0 .0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 0w a ve le n g th (n m )05 0 0 0 01 0 0 0 0 01 5 0 0 0 02 0 0 0 0 02 5 0 0 0 03 0 0 0 0 03 5 0 0 0 04 0 0 0 0 04 5 0 0 0 05 0 0 0 0 05 5 0 0 0 06 0 0 0 0 06 5 0 0 0 07 0 0 0 0 07 5 0 0 0 08 0 0 0 0 08 5 0 0 0 09 0 0 0 0 09 5 0 0 0 01 0 0 0 0 0 0Intensity3 9 3 .2 53 0 9 .2 23 9 4 .2 84 7 7 .1 46 5 5 .4 64 1 5 .3 12 8 1 .2 65 1 7 .0 43 6 3 .0 57 5 5 .1 16 4 2 .5 15 8 4 .4 86 7 0 .7 67 2 3 .0 43 1 0 .3 75 5 3 .2 54 7 5 .0 06 1 0 .5 72 7 9 .3 52 2 3 .1 91 9 1 .0 1OOHOHOHNNOOHOHHNONp y r i d i n e # 2 4 9R T : 9 .4 8A V : 1N L : 3 .3 5 E 5T : - c E S I F u l l ms 1 5 0 .0 0 - 1 5 0 0 .0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th ( n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 02 6 0 0 0 02 8 0 0 0 03 0 0 0 0 03 2 0 0 0 0Intensity3 9 3 .2 08 0 9 .2 08 1 0 .1 54 1 5 .1 53 7 8 .2 57 6 5 .8 48 3 3 .4 56 8 9 .3 93 6 2 .3 06 6 8 .1 53 0 9 .1 78 8 1 .5 75 3 1 .1 57 6 8 .5 14 9 4 .7 64 2 3 .1 96 0 6 .8 77 2 4 .9 38 0 8 .3 25 6 2 .6 33 3 1 .0 12 7 2 .1 82 3 3 .1 41 8 9 .0 2p y r i d i n e # 1 1 3R T :4 .2 8A V :1N L :4 .0 2 E 5T :- c E S I F u l l ms 1 5 0 .0 0 - 1 5 0 0 .0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th ( n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 02 6 0 0 0 02 8 0 0 0 03 0 0 0 0 03 2 0 0 0 03 4 0 0 0 03 6 0 0 0 03 8 0 0 0 04 0 0 0 0 0Intensity4 0 9 .1 84 4 3 .2 65 4 7 .1 83 9 1 .0 95 2 5 .1 13 3 9 .2 47 7 9 .3 34 5 3 .2 77 3 7 .1 35 1 3 .1 37 1 8 .5 33 5 2 .9 48 1 9 .3 32 5 3 .2 95 6 6 .7 06 3 9 .0 55 0 8 .9 83 1 1 .1 86 5 4 .3 28 4 3 .1 28 8 4 .5 62 0 2 .1 22 3 0 .1 2p y r i d i n e # 3 1 7R T : 1 2 .0 9A V : 1N L : 2 .6 0 E 5T : - c E S I F u l l ms 1 5 0 .0 0 - 1 5 0 0 .0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th ( n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 0Intensity4 6 1 .2 03 6 1 .2 54 8 3 .0 54 0 5 .3 27 7 7 .0 94 0 6 .3 95 8 3 .0 95 6 5 .1 47 4 5 .8 77 0 6 .4 28 2 2 .7 13 9 3 .2 64 9 6 .5 96 4 3 .0 15 8 4 .2 14 3 8 .6 25 6 2 .4 68 6 9 .9 63 3 3 .1 66 9 7 .7 77 3 0 .6 97 8 8 .2 75 4 2 .8 73 2 2 .8 62 7 1 .1 11 8 3 .2 42 4 9 .0 5p y r i d i n e # 3 2 3R T : 1 2 .3 2A V : 1N L : 2 .8 1 E 5T : - c E S I F u l l ms 1 5 0 .0 0 - 1 5 0 0 .0 0 1 5 02 0 02 5 03 0 03 5 04 0 04 5 05 0 05 5 06 0 06 5 07 0 07 5 08 0 08 5 09 0 0w a v e l e n g th ( n m)02 0 0 0 04 0 0 0 06 0 0 0 08 0 0 0 01 0 0 0 0 01 2 0 0 0 01 4 0 0 0 01 6 0 0 0 01 8 0 0 0 02 0 0 0 0 02 2 0 0 0 02 4 0 0 0 02 6 0 0 0 02 8 0 0 0 0Intensity3 6 1 .2 14 6 3 .1 64 6 1 .1 18 7 7 .1 84 6 4 .2 38 4 4 .9 16 9 2 .8 34 9 7 .0 63 6 3 .2 94 0 5 .6 35 4 5 .0 28 0 9 .2 86 6 2 .9 85 8 9 .5 17 4 4 .8 93 8 3 .0 68 7 9 .2 03 4 3 .3 24 2 2 .5 96 4 1 .0 95 0 6 .7 67 1 0 .7 03 2 3 .2 68 9 5 .5 52 7 2 .9 91 8 3 .3 62 4 1 .0 6 感谢导师马志玲副教授的悉心指导 感谢吴京洪、凌连生 等老师的关心 感谢师兄师姐对我的鼓励和热心帮助感谢我的同学对我的照顾和支持