【病毒外文文献】2013 Full-length infectious cDNA clone of Middle East respiratory syndrome coronavirus (MERS-CoV)

the distillery SciBX Science Business eXchange Copyright 2013 Nature Publishing Group 1 This week in techniques Approach Summary Licensing status Publication and contact information Disease models Full length infectious cDNA clone of Middle East respiratory syndrome coronavirus MERS CoV A red fluorescent protein RFP expressing recombinant full length infectious cDNA clone of MERS CoV could be used to characterize viral pathogenesis and screen for antivirals Reverse genetic strategies generated a MERS CoV that lacked open reading frame 5 and encoded RFP The RFP expressing MERS CoV replicated to high levels in several primary lung cells including fibroblasts type II pneumocytes and nonciliated human airway epithelial cells and could be quantified by fluorescence Ongoing studies include using the MERS CoV clone to test compounds for antiviral activity and have identified hits that show broad based activity against viral strains including MERS CoV SciBX 6 41 doi 10 1038 scibx 2013 1171 Published online Oct 24 2013 Reverse genetic strategies for SARS mouse hepatitis virus and other coronaviruses including MERS CoV are patented unlicensed Scobey T et al Proc Natl Acad Sci USA published online Sept 16 2013 doi 10 1073 pnas 1311542110 Contact Ralph S Baric The University of North Carolina at Chapel Hill Chapel Hill N C e mail rbaric ad unc edu