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1,肝脏肿瘤的课题报告,2,三方面： 1、肿瘤干预手段 2、肿瘤微环境 3、肿瘤遗传背景,3,三方面： 1、肿瘤干预手段 2、肿瘤微环境 3、肿瘤遗传背景,4,收集肝癌肝移植样本临床资料 根据肝癌肝移植术后肿瘤复发情况分组 比较组间免疫组化、基因测序，分析数据 得出结论 验证,5,三方面： 1、肿瘤干预手段 2、肿瘤微环境 3、肿瘤遗传背景,6,Mutation,7,Biomarker,8,ncRNAsSmall ncRNAs(200 nt)microRNAs(miR-221,miR-133a)siRNAspiRNAsLong ncRNAs,9,10,11,12,HOTTIP/HOXA13(guide),13,Patient Samples(n=52)All patients in this study met the following criteria: HCC diagnosis was verified by pathological examination, no anticancer treatments were given before biopsy collection, and exhaustive clinical-pathologic and follow-up data were available. The data were obtained from snap-frozen needle biopsies samples collected from patients who did not receive any HCC-tailored therapeutic treatments prior to the biopsy. This increases the value of our work, in contrast to the majority of similar studies employing surgical-resected specimens often exposed to ischemic or therapy-induced damage,14,Methods: Relative Expression of HOXA13 and HOTTIP by qRT-PCR Proliferation, Migration, and Apoptosis Assay Cell Lines Small Interfering RNA (siRNA) Transfection and HOXA13 Over expression,15,They screened the entire HOX gene network expression levels in a series of liver biopsies demonstrating that the HOXA locus represents the prevalent locus involved in hepatocarcinogenesis. Within HOXA genes, we have identified HOXA13 as the most up-regulated in HCC. They performed qRT-PCR analysis on total RNA extracted from 52 HCC liver needle biopsies and their matched nonneoplastic counterpart. In addition, we evaluated HOTTIP and HOXA13 expression in 30 liver biopsies obtained from patients with nonneoplastic liver disease including: steatosis (n = 6), liver inflammation (total of n = 6, of which n = 2 drug-induced liver hepatitis plus n = 4 nonalcoholic steatohepatitis), alcohol and virus related cirrhosis (n = 5), HCV infection without signs of cirrhosis (n = 4), HCV infection with signs of cirrhosis (n = 5), and normal liver donors (n = 4).,16,Conclusion: high levels of HOTTIP/HOXA13 expression are associated with metastasis formation and predict HCC patients clinical outcome. Future work will validate HOTTIP as a predictive biomarker for HCC development. A deeper characterization of the function and downstream signaling pathways influenced by HOTTIP and HOXA13 deregulation may provide novel insights into the mechanisms of hepatocarcinogenesis, possibly leading to the development of new therapeutic agents.,17,Thanks！,
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