资源描述
,Gaoyan,ImageJFeatures,RunsEverywhere:ImageJiswritteninJava,whichallowsittorunonLinux,MacOSXandWindows,inboth32-bitand64-bitmodes.OpenSource:ImageJanditsJavasourcecodearefreelyavailableandinthepublicdomain.Nolicenseisrequired.Plugins:ExtendImageJbydevelopingpluginsusingImageJsbuiltintexteditorandJavacompiler.Morethan500pluginsareavailable.Analysis:Measurearea,mean,standarddeviation,minandmaxofselectionorentireimage.Measurelengthsandangles.Userealworldmeasurementunitssuchasmillimeters.Calibrateusingdensitystandards.Generatehistogramsandprofileplots.,Installation,LinuxMacOSXMacOS9Windows,Windowshttp:/imagej.nih.gov/ij/download.htmlDownloadImageJ1.44bundledwith32-bitJava1.6.0_20(28MB),with64-bitJava1.6.0_20(24MB;requires64-bitWindows)orwithoutJava(3MB).,OpenReadsanimageanddisplaysitinaseparatewindow.FilesmustbeinTIFF,GIF,JPEG,DICOM,BMP,PGMorFITSformat.AlsoopensImageJandNIHImagelookuptables(with.lutextension).AdditionalfileformatsaresupportedviapluginsinstalledintheImportsubmenu.,Subtractbackground,Mergechannel,Scalebar,Scalebarsshouldbepresentonallpublication/presentationimages/movies.Itsworthputtingtheminsoonerratherthanlater.Chooseastandardscalebarsizeforallyourimagesifpossibletoavoidconfusion.,Ifyouknowthesizeofafeature(previouslyappliedscalebarforinstance)youcanusethiscommandtoapplyacalibration.Usingthelineselectiontool,drawalingalongthelengthofthefeature/scalebar.Runthemenucommand“Plugins/Spatialcalibration/Setscale”1.Enterthedimensionsoftheobject/scalebarinthe“knowndistance”boxandsettheunitsintheUnitoflengthbox.DonotcheckGlobalunlessyouwishallyourimagestohavethiscalibration!ClickOK.,AnalyzeParticlesandcountcell,Thiscommandcountsandmeasuresobjectsinbinaryorthresholdedimages.Itworksbyscanningtheimageorselectionuntilitfindstheedgeofanobject.Itthenoutlinestheobjectusingthewandtool,measuresitusingtheMeasurecommand,fillsittomakeitinvisible,thenresumesscanninguntilitreachestheendoftheimageorselection.Presstheesckeytoabortthisprocess.UseImageAdjustThresholdtothresholdanimage.,Selectionandcrop,Thankyou!2011-4-19,
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