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Click to edit Master text styles,Second level,Third level,Fourth level,Fifth level,*,Click to edit Master title style,*,Trends and Needs in Food Diagnostics,Larissa Kahr-BIT,Austria,Malin Brnnback-TEKES,Finland,Gabriela von Blankenfeld-Enkvist-TEKES,Finland,Aim of the Study,Investigate technological trends and needs in Food Diagnostics,Understand the factors that shape and influence the development of this field.,2,Focus,Background on food safety management tools,Description of the methods currently used and new methods developed,The market for food diagnostics,3,Definitions,Food Diagnostics applies modern methods developed in human,in vitro,diagnostic to food,Rapid Methods comprises different types of tests,Miniaturised biochemical kits,Antibody-based tests,Nucleic acid hybridisation-based tests,Manual,semi,-automated or fully automated,4,Detection,Bacteria,viruses,parasites,chemicals,biotoxins,heavy metals and prions,In all steps of the,food chain,-from raw materials to end products,5,Food as test matrix,Extremely varied in its chemical composition,Contains many different ingredients,Might contain an intrinsic microbial flora,6,Frame Food Safety,Process control is more efficient than product control,The safety of a product with low defect rates cannot be guaranteed through sampling and testing,The focus is changing from end-product testing to,HACCP,This has to be considered when developing new methods within the conceptual frame of,food safety,7,Microbiological methods can be divided into“traditional”or“conventional”methods and rapid methods,8,Conventional Methods,Conventional methods rely on culturing microorgansims onto agar plates,Laborious and time-consuming,84%of all tests arebased on counting viable cells,Required by national and international regulatory agencies for official control,9,Rapid Methods,Advantages in analysis time,Possibility to eliminate labour intensive steps,Potential for automation,However,lengthy incubation procedures are still necessary,First one-shift methods under development,ELISA and PCR are dominating,Lack of a common validation procedure,Mostly not accepted for official control purposes,10,Methods,Advances in Viable Cell Count Methods,Miniaturisation and Diagnostic Kits,Biochemical Identification Techniques,Antibody-based methods,Immunomagnetic Separation,Nucleic acid-based assays,Biosensors,Microarrays,Flow Cytometry,Bacteriophage-Based Techniques,ATP bioluminescence,Adenylate kinase bioluminescence,Riboprinting and Pulse-Field Gel Electrophoresis,11,Viable cell counting,Will remain important for the assessment of safety and quality of food products,development of semi-automated alternative methods,new methods are based on growth and metabolic activity,Microscopic methods,flow cytometry and cell sorting(FACS),12,Immunoassays,ELISA(with dipstick technology),Immunochromatography,Fully automated systems already on the market,New recombinant antibodies and molecular imprinting techniques to improve sensitivity and versatility,13,Immunomagnetic Separation,Speed up enrichment steps,No detection method itself,Combination with different end-detection methods to improve,-Speed and sensitivity,-Sample throughput,-User friendliness,-Automation expected in the next future,14,Nucleic acid based assay,Directly or after amplification with PCR,Development of homogenous assays and automation improves routine use and reduces false positive results after PCR,New assay formats under development,15,Biosensors and Microarrays,On-line or on-site,sensitive,low-cost,rapid methods for routine-use,Prototypes under development,Have high potential for automation,Simple and portable equipment can be designed,Applications within quality and process control,control of fermentation processes,quality and safety control of raw materials and for HACCP monitoring,16,Hygiene monitoring,Need to develop real time methods to monitor cleaning procedure,Methods should be cheap,robust,ATP bioluminescence,Adenylate kinase,biosensor applications might be further developed,17,Methods Implementation,In house,alternative methods for viable cell counts,hygiene monitoring,chemical analysis,Outsourcing,pathogen testing,18,The Market for Food Diagnostics,Definitions,Food Analysis:all food testing,Food Diagnostics:food testing with rapid methods,Microbiological Testing:all testing for microorganisms,Food Microbiological Testing:all testing for microorganisms in food and beverages,19,The Market for Food DiagnosticsSome figures,Food analysis global market 1.1 billion,Shared by about 50 companies,USA and Europe make up about 2/3,565 million global sales for Food microbiological tests,Rapid methods 115 million or a 20%total market share,20,Technology,Sales($US millions),Market Share(%),ELISA/EIA,70,61,DNA,25,22,Impedance,10,9,Flow Cytometry,5,4,ATP,5,4,Total,115,100,21,Food Analysis,(million),Food Diagnostics(million),%Food Diagnostics,(million),Food pathogens,97,5,13,15%,Spoilage,220,37,5,17%,Chemical contaminants,53,19,5,40%,Pro
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