资源描述
单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,Excitatory effect of oxytocin on uterine smooth muscle,Shi-Hong Zhang,（张世红）,Dept of P,Experimental pharmacology of reproductive system,Excitatory effect of oxytoc,Pituitrin(,垂体后叶素,),Oxytocin(,催产素,),Ergot alkaloids(,麦角生物碱,),Prostaglandins(PGs,前列腺素,),Uterine stimulants,Pituitrin(垂体后叶素)Uterine stim,Pituitrin(,垂体后叶素,),oxytocin,vasopressin,Uterine stimulants,垂体,Pituitrin(垂体后叶素)oxytocin Ute,Oxytocin(OXT,催产素,),1.Pharmacological effects:,(1)Uterus:,Elevating both the,frequency,and,force,of uterine contraction,at rhythm similar to natural labor,highly dependent on,estrogen level and condition of uterus,.,(2)Breast:,milk ejection.,(3)Blood vessel:,vasodilation at large dose.,Uterine stimulants,Oxytocin(OXT,催产素)1.Pharmaco,2.Clinical uses:,-to expedite delivery and induced labor;,-to reduce the incidence and severity of postpartum hemorrhage.,Uterine stimulants,Oxytocin(OXT,催产素,),2.Clinical uses:Uterine stimu,3.Adverse effects:,-Intrauterine asphyxia or uterine rupture(overdose),-Allergy,4.Contraindications:,-,hypertonic dysfunction of uterus,-abnormality in birth canal,-abnormal fetal position,Uterine stimulants,Oxytocin(OXT,催产素,),3.Adverse effects:Uterine sti,Ergot alkaloids(,麦角生物碱,),Ergometrine(,麦角新碱,),Ergotamine(,麦角胺,),Ergotoxine(,麦角毒,),1.Pharmacological effects:,(1)Uterus,(ergometrine),:,-strongly stimulating uterine smooth muscle,both the body and the cervix;ready to induce tonic contraction at larger dose.,(2)Blood vessel,(ergotamine),:,-contracting peripheral blood vessels and c,erebral vessels,.,(3)blocking,receptor,(ergotoxine),:,Uterine stimulants,Ergot alkaloids(麦角生物碱),2.Clinical uses:,(1)Endometrorrhagia(,子宫出血,),and puerperal replacement of uterus(,产后子宫复位,),:,ergometrine,;,(2)Migraine(,偏头痛,):,ergotamine,;,(3)Hibernation therapy(,冬眠疗法,):dihydro,ergotoxine+pethidine+promethazine.,A.Uterine stimulants,Ergot alkaloids(,麦角生物碱,),2.Clinical uses:A.Uterine st,3.Adverse effects:,-nausea,vomiting,BP,;,-allergic reaction;,-prohibited in expediting delivery and induced labor.,A.Uterine stimulants,Ergot alkaloids(,麦角生物碱,),3.Adverse effects:A.Uterine,Prostaglandins(PGs,前列腺素,),Dinoprostone(,地诺前列酮,PGE,2,),Dinoprost(,地诺前列素,PGF,2,),1.Pharmacological effect:,stimulating uterine smooth muscle.,2.Clinical uses:,to,induce labor,2.Adverse effects:,GI reaction,attack of asthma(PGF,2,),;glaucoma(PGE2).,Uterine stimulants,Prostaglandins(PGs,前列腺素),Objectives,To observe the excitatory effect of oxytocin on uterine smooth muscles and its dose dependent manner.,ObjectivesTo observe the excit,Methods,Estrodiol pretreatment(0.2mg)on rats(200 g,40g,)24hr before experiment;,Prepare uterus smooth muscle specimen.,MethodsEstrodiol pretreatment,Methods,Uterus of the rat,MethodsUterus of the rat,Methods,Uterus of the rat,MethodsUterus of the rat,Methods,Perfusion system,MethodsPerfusion system,Methods,Set parameters of recording system:,Input pattern:tensility,Gain:200-500,Lower frequency:direct current,Upper frequency:10 Hz,Sampling interval:2 ms,Preload:1g,MethodsSet parameters of recor,Methods,The specimen is stabilized in the perfusion chamber for 15-30 min until the contractive force and rhythm become regular.Record the basal contractive force and frequency(optional).,Add 0.02 ml,0.04 ml,0.14 ml oxytocin,(0.01U/ml),into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.,MethodsThe specimen is stabili,Methods,Add 0.04 ml,0.14 ml oxytocin,(0.1U/ml),into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.,Add 0.04 ml,0.14 ml oxytocin,(1U/ml),into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.,MethodsAdd 0.04 ml,0.14 ml ox,Methods,Add 0.04 ml,0.14 ml oxytocin,(10U/ml),into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.,MethodsAdd 0.04 ml,0.14 ml ox,Methods,Notice:,1,Avoid injury and excessive stretch of uterus during the whole process of preparation.,2,Control the gas bubbles in a proper rate in case of impact on uterus contraction.,3,Record a period of baseline of contraction before drug treatment in order to compare that before and after.,4,Add drug in the order as prescribed.,MethodsNotice:,Lab report,1,题目,2,署名,:,作者和合作者姓名及单位。,3,结构式摘要（目的，方法，结果，结论）。,4,材料和方法,:,主要实验材料，标本制备方法，仪器装置连接和仪器参数，实验数据记录条件，观察指标。,5,结果,:,实验结果的文字性描述，作量效曲线。,6,讨论,:,回答思考题。,Lab report1题目,L,earning issue,1,缩宫素对子宫的作用,及,特点。,2,为什么实验前用雌二醇处理动物？,3.,缩宫素与麦角新碱对子宫平滑肌的作用特点有何不同？,Learning issue1缩宫素对子宫的作用及特点。,
展开阅读全文