血液和造血(英文版)ppt课件

,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,Blood and Hemopoiesis,Blood and Hemopoiesis,1.,Components:,-formed elements: 45%,red blood cell-,erythrocyte,white blood cell-,leukocyte,platelets,1. Components:,-plasma: 55%,PH 7.3-7.4,90% water,contain:,plasma protein (albumin, globulin, fibrinogen),lipidprotein,enzymes,hormone,vitamin,inorganic salt and products of metabolism,*,serum:,plasma without fibrinogen,-plasma: 55%, PH 7.3-7.4,Blood smear: Wright or Giemsa stain methods,Blood smear: Wright or Giemsa,1.Cells,1.Cells,1),erythrocyte,red blood cell,-normal number:,(4.2-5.5)X10,12,/L in male,(3.5-5.0)X10,12,/L in female,1) erythrocyte, red blood cell,-structure:,biconcave discs in shape,7.5-8.5 um in diameter, 1-2um thick,no nucleus and organella,filled with hemoglobin (,Hb,),-normal,Hb,:,120-150g/L in male,105-135g/L in female,-Function:,combine and transfer,Oxygen (O,2,) and Carbon dioxide(CO,2,),-structure:,*,anemia: RBC 3.0X10,12,/L or Hb 100g/L,*anemia: RBC 3.0X1012 /L or H,ABO blood type,blood type antigen: A, B :,-membrane protein,hemolytic transfusion reaction,-hemolysiserythrocyte ghost,ABO blood type,ABO Blood Group System:,ABO Blood Group System:,血液和造血(英文版)ppt课件,*,reticulocyte,:,immature cell,remained ribosome: delicate network structure when stained with brilliant cresyl blue,0.5-1% of total erythrocyte population,or 3-6% in neonate,-life span: 120 days,血液和造血(英文版)ppt课件,2) leukocyte,-a group of large cells with nucleus,-involve in defense and immune reaction,-normal number: (4.0-10) X10,9,/L,-classification:,granulocytes:,/neutrophil,/eosinophil,/basophil,agranulocytes:,/lympocyte,/monocyte,2) leukocyte,neutrophil,:,50-70% of total leukocyte population,-structure:,LM:,round, 10-12um in diameter,rob-liked or poly morphous nucleus, 2-5 lobes,fine neutrophilic granules-pink cytoplasm,血液和造血(英文版)ppt课件,EM:,specific granule,:,80%,small, 0.3-0.4 um,ovoid or irregular in shape,contain: lysozyme, phagocytin ,AKPase,ect.,azurophilic granule,:,20%,large, round or ovoid , 0.6-0.7 um,electron dense,acid phosphatase, peroxidase, ect.,血液和造血(英文版)ppt课件,-function:,phagocytose bacterium:,specific g.-kill B,azurophilic g.- digest B,-life span:,in blood 6-8 hours, in CT 2-3 days,-function: phagocytose bacte,血液和造血(英文版)ppt课件,Neutrophilic granulocyte,Neutrophilic granulocyte,Neutrophilic granulocyteNeutro,eosinophil,:,0.5-3% of total leukocyte population,LM:,round,10-15 um in diameter,2 lobes nucleus,rough bright red granules -acidophilic granules,血液和造血(英文版)ppt课件,EM: granules:,round or ovoid,with cube-liked electron dense crystal,contain:,-ACPase,-histaminase,-peroxidase,EM: granules:,-function:,counteract the infection of parasite,reduce allergic reaction,-life span:,in blood 6-8 hour, in CT 8-12 days,-function:,血液和造血(英文版)ppt课件,Eosinophilic granulocyte,致密结晶体,Eosinophilic granulocyte致密结晶体,basophil,:,0-1% of leukocyte population,LM:,less, round 10-12 um,lobe, “S” shaped or irregular nucleus,basophilic g.: contains heparin, histamine and acidophil chemotactic factor,cytoplasm: contain leukotriene,-function:,involve in allergic reaction,-life span: 10-15 days, basophil:,血液和造血(英文版)ppt课件,Basophilic granulocyte,Basophilic granulocyte,monocyte:,3-8% of total leukocyte population,LM:,large round, 14-20um,nucleus appear as kidney, horse-shoe or ovoid in shape,cytoplasm: gray-blue in color, contain azurophilic g., monocyte:,EM:,azurophilic granule:,peroxidase, ACPase, lysozyme,-,function:,actively mobile and chemotaxis,-,life span:,in blood 1-5 days, in CT which become into macrophage- mononuclear phagocytic system,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,lymphocyte,:,20-30% of total,leukocyte population,LM:,round,small,LC 6-8 um,medium-sized,LC 9-12 um,large,LC 13-20 um in diameter,round nucleus with indentation, chromatin appears as spot-liked and electron-dense,cytoplasm: /basophilic:bright blue in color,/less,/azurophilic granule, lymphocyte:,EM: /free ribosome,/mito.,/RER,-classification:,TC:,thymus dependent lymphocyte, 75%, involve in cellular immune reaction and regulate immune response,BC:,bone marrow dependent lymphocyte, 10-15%, become into plasma cell, involve in humoral immune response,large granular cell:,KC (killer cell) and NKC( nature killer cell), 10%,-function:,involve in immune response,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,Lymphocyte,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,3),blood platelet,:,cytoplasmic fragment of megakaryocyte in bone marrow,-normal number: 100-300X10,9,/L,-structure:,LM:,disc-liked, 2-4um irregular in shape,Stimulation,in groups,Granulomere,hyalomere,3) blood platelet:,EM:,/tubular systems,:,opening tubule system-,increasing the exchange area, facilitate the intaking and releasing,dense tubule system-,SER, collecting calcium,(,Ca,2+,) and synthesizing,prostaglandin,/granules,:,specific granule:,mediate electron density, with dense core, contains,clotting factor,fibrinogen, acidic hydrolase,dense granule:,electron dense, contains 5-hydroxytryptamine, ADP, ATP, Ca,2+,and adrenalin,EM:,血液和造血(英文版)ppt课件,-function:,involve in clotting and stopping the bleed:,-aggregation,-release components of granule, make thrombinogen become into thrombin, then later make fibrinogen become into fibrin,protecting endothelium and take part in repairing of endothelium,-function:,血小板（EM）,Blood platelet,特殊颗粒,血小板（EM）特殊颗粒,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,血液和造血(英文版)ppt课件,3.,Bone marrow and hemopoiesis,Places for hemopoiesis and structure of bone marrow,Places for hemopoiesis,:,yalk sac ( 3W)liver(6W) spleen(4M) bone marrow,3.Bone marrow and he, Structure of bone marrow,:,red bone marrow,-hemopoietic tissue:,reticular T.,hemopoietic cell, macrophage, fibroblast, mesenchymal cell,-sinusoid: cap.,血液和造血(英文版)ppt课件,*,hemopoietic inductive microenvironment,( HIM):,The environment for hemopoietic cell growth and development, bone marrow HIM includes N of bone marrow, BV, fiber, extracellular GS and hemopoietic stromal cells,*hemopoietic inductive microen,* stromal cells,:,-including,reticular cell,fibroblast, macrophage, endothelial cell and lipid-laden cell,-function:,supporting,secrete cellular factors, regulate the proliferation and differentiating of hemopoietic cells,* stromal cells:,2) Hemopoietic stem cell and hemopoietic progenitor cell,hemopoietic stem cell (HSC,): multipotential stem cell,-originated,yalk sac red bone marrow constitutes about 0.5% of total bone marrow cells,similar to small LC,-feature:,strong potential to proliferation multidifferentiated ability,ability to copy itself: keep certain number,2) Hemopoietic stem cell and h,hemopoietic progenitor cell (HPC),:,committed stem cell,-originated from hemopoietic stem cell,-forming,colony forming unit,(CFU) under the regulate of colony stimulating factor (CSF),hemopoietic progenitor cell (,3) General regulation of hemopoietic processes:,-main steps:,primordial stage,immature stage,mature stage,3) General regulation of hemop,-,regulations:,size of cell become smaller and smaller,Nucleus become smaller and smaller or disappears,chromatin: from fine and loose to rough and dense,cytoplasm: -more and more,-basophilic: weaker and weaker,-specific granule: more and more,ability to division: from have to no, but keep the potentials,-regulations:,血细胞发生过程,血细胞发生过程,红细胞发生,红细胞发生,Proerythroblast,Proerythroblast,Early erythroblast,Early erythroblast,Intermediate erythroblast,Intermediate erythroblast,Late erythroblast,Late erythroblast,Reticulocyte,Reticulocyte,Erythrocyte,Erythrocyte,?,?,TEM,neutrophil,TEMneutrophil,?,?,TEM,monocyte,TEM monocyte,?,?,TEM,Eosinophilic granulocyte,TEMEosinophilic granulocyte,?,?,TEM,Lymphocyte,TEMLymphocyte,?,?,Basophilic granulocyte,Basophilic granulocyte,