【病毒外文文献】2017 PCR Array Profiling of Antiviral Genes in Human Embryonic Kidney Cells Expressing Human Coronavirus OC43 Structural

S312 OFID 2017 4 Suppl 1 Poster Abstracts Session 139 Adult Viral Infection Friday October 6 2017 12 30 PM Background Human coronaviruses HCoV OC43 229E NL63 and HKU1 commonly cause upper respiratory tract infections but can also cause severe lower respiratory tract disease Increased use of diagnostic assays for respiratory viruses has facilitated detection and since 2014 voluntary reporting of HCoV to the National Respiratory and Enteric Virus Surveillance System NREVSS Methods We reviewed weekly aggregate test results for HCoV OC43 229E NL63 and HKU1 voluntarily reported to NREVSS by U S hospital and clinical lab oratories from July 1 2014 April 30 2017 Laboratories reporting any HCoV result using PCR were included and the weekly percentage of positive HCoV tests by type was calculated For a subset of HCoV detections reported to NREVSS via the Public Health laboratory Interoperability Project PHLIP which collects individual level demographic data we described age distribution and sex Age distribution by HCoV type was compared using the Kruskal Wallis test Results 154 laboratories across all 9 U S census divisions reported 834 742 tests for HCoV 18 514 2 2 were positive for HCoV OC43 8 363 1 0 for HCoV NL63 6 828 0 8 for HCoV 229E and 5 170 0 6 for HCoV HKU1 The percentage of tests positive for HCoV generally peaked between December and March Figure 1 HCoV OC43 showed distinct annual peaks with variation in magnitude by year HCoV HKU1 and NL63 had similar patterns each with notable peaks during winter 2016 compared with 2015 or 2017 HCoV 229E showed a discernable peak in 2017 compared with the previous 2 years Of 20 533 individuals with HCoV test results reported via PHLIP 1 589 7 7 tested positive for any HCoV 50 of HCoV positive individuals were male and the median age was 22 range 0 96 years Age distribution differed between HCoV types P 18 years presenting with respiratory illness in Cleveland Ohio Methods Between February 1 2016 and April 30 2017 2949 nasopharyngeal swab specimens were analyzed by NxTAG Respiratory Pathogen Panel in adults pre senting with respiratory illness at MetroHealth Medical Center Clinical data were collected on adults whose samples screened positive for CoV HKU1 CoV OC43 CoV 229E or CoV NL63 Results Coronaviruses were detected in 192 6 5 adults including 105 3 5 OC43 67 2 3 229E 13 0 4 HKU1 and 7 0 2 NL63 The majority of adults with coronavirus infection were females 66 2 with a median age of 53 years Common comorbidities included smoking 40 0 asthma 38 0 COPD 35 4 and inhaled corticosteroid use 28 6 Eighty five 46 4 required admission to the hospital Common presenting symptoms included shortness of breath 42 7 and cough 31 0 whereas fever was uncommon 12 5 Gastrointestinal symptoms were more common in HKU1 and NL63 infected adults Seventy three percent of coronavirus disease occurred between the months of January and March Despite the recognition of coronavirus infection 70 36 5 received antibiotics for their disease Conclusion This study provides needed insight into clinical characteristics and severity associated with coronavirus infection in adults Coronavirus infection should be considered in differential diagnosis of respiratory tract illness in adults including those that require hospitalization have a history of smoking and have pulmonary comorbidities Disclosures All authors No reported disclosures 1033 Evaluation of Serum TNF alpha IL 6 IL 10 and IFN gamma Levels in Patients with Crimean Congo Hemorrhagic Fever H lya Yilmaz MD 1 G rdal Yilmaz Professor 2 Ugur Kostakoglu Assistant profes sor 3 Mustafa Arslan MD 4 S leyman Caner Karahan Professor 5 and Iftihar Koksal Professor 2 1 Department of Medical Biochemistry Health Sciences University Kanuni Training and Research Hospital Trabzon Turkey 2 Department of Infectious Diseases and Clinical Microbiology Karadeniz Technical University Medical Faculty Trabzon Downloaded from by guest on 17 October 2018