【病毒外文文献】1992 New recombinant adenovirus used as vaccine_ dog adenovirus-2 vector_ dog parvovirus, cat panleukopenia virus, cat o

Patent Report This section provides information on worldwide patents relevant to vaccine design and production The Patent Report gives the following information title of patent patentee patent number publication date and summary of the patent A number of patents in this report are reproduced from Biotechnology Abstracts with permission of Derwent Publications Ltd Rochdale House 128 Theobalds Road London WC1X 8RD Immunologically active parvovirus BI9 peptide comprises capsid protein VP1 or VP2 fragment application to recombinant vaccine construction and disease diagnosis protein sequence Mikrogen Mol Biol 4003 826 14 August 1991 New immunologically active peptides I comprise part of the amino acid sequence of capsid protein VP1 or VP2 of parvovirus B19 and are free of impurities that may interfere with the detection of B19 specific antibodies Specified DNA sequences are used as DNA primers for the direct detection of the parvovirus B 19 pathogen by DNA amplification especially by the polymerase chain reaction I include VP1 fragments designated PCE and PAN 1 to PAN 4 specified protein sequences VP2 fragments designated PANSE and PAPST specified protein sequences and the small fragments PAPEP 1 to PAPEP 8 The VP1 and VP2 fragments are expressed by plasmid pUC12PAN and plasmid pUC12VP2 respectively which contain DNA sequences generated from virus DNA using the new DNA primers in pairs I may be used in the development of a vaccine against parvovirus B19 infections and as immunoassay reagents for detection of anti B19 antibodies e g for diagnosis of BI9 infections 009 92 Feline T lymphotropic lentivirus FTLV separated from cell culture and useful as vaccine hybridoma construction and monocional antibody preparation for use in immunoassay DNA probe construction and HIV virus model system Univ Calif USA 5037 753 6 August 1991 Feline T lymphotropic lentivirus FTLV isolated from cells grown in in vitro cell culture is new The virus is specifically characterized by a Western blot shown in the specification Also new is a biologically pure culture of FTLV strain Petaluma ATCC VR 2186 The virus or its components e g major envelope and core proteins or antibodies raised against them can be used to detect and vaccinate against the new feline retrovirus The virus will also serve as a useful model for HIV virus and other mammalian retroviruses The FTLV may be produced by cultivation of e g peripheral blood lymphocytes or lymph nodes isolated from the blood of infected cats Genes encoding the desired FTLV protein or fragment may be isolated or produced synthetically and expressed in bacterial yeast or mammalian hosts Monoclonal antibodies specific for the FTLV proteins can be obtained by conventional hybridoma technology and are useful in immunoassays DNA or RNA probes can also be prepared for use in detecting the presence of FTLV in biological samples 010 92 New recombinant adenovirus used as vaccine dog adenovirus 2 vector dog parvovirus cat panleukopenia virus cat or dog coronavirus dog distemper virus cat leukemia virus rabies virus or FIV virus recombinant vaccine construction Univ Glasgow World 9111 525 8 August 1991 A recombinant adenovirus vector for producing antibodies or cell mediated immunity to infection in carnivores is new The vector is a live non pathogenic immunogenic viable dog adenovirus 2 vector modified to contain an antigen gene in association with a promoter The promoter sequence may be introduced into a viral genomic region from the Sinai site close to the end of the inverted terminal repeat to the early region four promoter preferably at the SmaI site The antigen gene may encode a dog parvovirus capsid protein a cat panleukopenia virus capsid protein a dog or cat coronavirus peplomere a dog distemper virus hemagglutinin or capsid antigen a cat leukemia virus envelope glycoprotein a rabies virus glycoprotein or an FIV virus glycoprotein The recombinant virus may be associated with dog adenovirus Ela proteins and may be replicated by transfection into a cell line expressing dog adenovirus Ela proteins Using the vector vaccines against a wide variety of diseases may be produced and the gene is inserted without disturbing reproduction of the adenovirus vector 011 92 New protein comprising at least one hepatitis A virus epitope capsid protein expression in vaccinia virus vector for use as recombinant vaccine Imperial Coll Sci Technol Med World 9111 460 8 August 1991 The following are claimed 1 an isolated protein which is an antigen comprising at least a part of a hepatitis A virus HAV epitope free from infectious material 2 protein 1 com prising at least part of HAV capsid proteins a VP1 b VP3 c VP3 and at least part of VP1 d VP4 VP2 VP3 and at least part of VP1 or d at least two of VP1 VP2 VP3 and VP4 3 proteins as in 2 conjugated to at least part of vaccinia virus thymidine kinase EC 2 7 1 21 4 a DNA sequence encoding the proteins of 2 operably linked to a vaccinia virus promoter 5 a genetically engineered vaccinia virus expressing proteins 2 under control of the vaccinia virus promoter and 6 a recombinant vaccine used to immunize HAV and comprising a protein 2 or recombinant vaccinia virus vector expressing 2 in association with a pharmaceutically acceptable carrier The proteins 2 expressed by vaccinia virus can evoke a protective immune response against HAV following a single injection The recombinant proteins can be used in the production of polyclonal or monoclonal antibodies for use in passive immunization or as diagnostic agents 012 92 Peptides from merozoite stage of malaria parasite and DNA sequence encoding them Plasmodium falciparum merozoite thrombospondin related anonymous protein expression in Spo doptera frugiperda insect cell transformed with nuclear poly bedrosis virus vector vaccine Res Exploit World 9111 516 8 August 1991 Proteins comprising amino acids 182 276 and 221 276 of a specified sequence are claimed The proteins represent the conserved and inner conserved sequences respectively of Plasmodium falciparum merozoite TRAP thrombospondin related anonymous protein Also claimed are a a DNA encoding the TRAP proteins b a recombinant vector containing the DNA of a where the vector is a plasmid phage M13mp8 plasmid pUCI3 plasmid pAcYM1 or a virus Autoyrapha californica nuclear polyhedrosis virus AcNPV c a host vector system capable of expressing the DNA of a and containing the vector of b where the preferred host is Spodoptera fruyiperda d preparation of the TRAP proteins 0264 410X 92 020133 2 1992 Butterworth Heinemann Ltd Vaccine Vol 10 Issue 2 1992 133