【病毒外文文献】2012 Epidemiology of the four human coronaviruses 229E, HKU1, NL63 and OC43 detected over 30 months in the Singapore mil

15th ICID Abstracts International Journal of Infectious Diseases 16S 2012 e2 e157 e135 Type Poster Presentation Final Abstract Number 41 029 Session Infectious Disease Surveillance Date Thursday June 14 2012 Time 12 45 14 15 Room Epidemiology NL63 tary C W J Chan Q H C 1 2 logical prevalence infection epidemiology washes with the of coronaviruses year 229E compare http dx doi org 10 1016 j ijid 2012 05 305 Type Final Session Date Time Room Burden Cruz in J Velez 1 2 3 4 lem DF prehensive dengue of utilization to pay WTP survey to determine the true burden of dengue in the Santa Cruz comuna of Medellin Colombia In the surveil lance febrile patients between 1 65 years of age are evaluated for dengue infection Among dengue confirmed cases the COI survey estimates the economic burden of disease For the sero survey 2000 randomly selected residents are enrolled to calculate sero conversion rate The WTP and the healthcare utilization surveys Poster sero survey a healthcare survey a cost of illness COI survey and a willingness assess private demand of dengue vaccines and the proportion of febrile cases missed by the passive surveillance Results Passive fever surveillance was launched in November 2011 in Santa Cruz Hospital and three affiliated health centers It has been a season of low caseload for Colombia with only 26 subjects enrolled until the 31st of Dec Among 23 samples processed 3 sam ples were found to be positive by IgM capture ELISA As there were none confirmed positive by NS1 rapid test the COI survey has not begun In the sero survey 61 n 1191 were found to be positive by IgG indirect ELISA among 1955 samples processed Prior to the WTP survey focus group discussions were conducted It was found that all respondents believe that dengue vaccines should be used and that the government should pay for children s vaccinations and cited price as the most important determinant of demand followed by efficacy duration and side effects A reasonable vaccine price was about 20 000 Colombian pesos per dose Conclusion The data generated by the DVI with epidemiolog ical economic behavioral market demand perspectives will be used to build a comprehensive national investment case of dengue vaccine in Colombia a likely early adopter country of dengue vaccines The investment case will be used as models for other countries in the respective regions to facilitate accelerated devel opment and introduction of safe and effective dengue vaccines http dx doi org 10 1016 j ijid 2012 05 306 Type Poster Presentation Final Abstract Number 41 031 Session Infectious Disease Surveillance Date Thursday June 14 2012 Time 12 45 14 15 Room Poster Exhibition Area Rapid differentiation of Vibrio cholerae strains using MLVA and creation of a public MLVA database for epidemiological investi gations J P Loh 1 W H V Koh 2 1 DSO NATIONAL LABORATORIES Singapore Singapore 2 DSO National Laboratories Singapore Singapore Background Vibrio cholerae is a globally important pathogen endemic in many areas of the world and causes up to 5 million reported cases of cholera every year Straintyping is thus important for epidemiological surveillance purposes as well as determining source of infections for more targeted preventive measures Mul tilocus variable number tandem repeat VNTR analysis MLVA is one method that has been proven useful for differentiating between strains that would be indistinguishable by other techniques such as PFGE and phage typing Methods Here we present a rapid method for strain typing of Vibrio cholerae using MLVA which does not require the use of a sequencer for analysis but give better resolution compared to conventional gel electrophoresis Results We are also in the process of generating a public MLVA database for Vibrio cholerae and compiling information from var ious sources and collaborators so as to build a useful database