【病毒外文文献】2007 Coronavirus NL63 Illnesses in Infancy are a Risk Factor for Asthma at Age Six

574 Coronavirus NL63 Illnesses in Infancy are a Risk Factor for Asthma at Age Six T E Pappas 1 K T Sullivan Dille 1 W Lee 1 K A Grindle 1 K A Ro berg 1 D F Da Silva 1 C J Tisler 1 E L Anderson 1 K M Hansen 1 R A Grabher 1 L E Pleiss Salazar 1 M D Evans 2 R Gagnon 2 J E Gern 1 R F Lemanske Jr 1 1 University of Wisconsin School of Medicine and Public Health Madison WI 2 University of Wisconsin Population Health Sciences Biostatistics and Medical Informatics Madison WI RATIONALE Ininfancy rhinovirusillnessesandsevere RSVbronchiol itis indicate increased risk for recurrent wheezing and asthma but there is less information about other viruses We evaluated the frequency and se verity of metapneumovirus HMPV and coronavirus HCV infections in a high risk group of infants METHODS Nasallavagesampleswereobtainedinthefirstyearoflifedur ing scheduled study visits and symptomatic respiratory illnesses as part of the Childhood Origins of Asthma Project COAST Samples n 5 567 were analyzed by Respiratory Multicode Assay RMA for HMPV HCV 229E HCV OC43 HCV NL63 and HCV SARS RESULTS HMPVwasisolatedin10 7 ofsamplesduringillnesses n5 291 and also occurred with other respiratory viruses 4 8 47 8 of HMPV illnesses included wheezing HCV were isolated in 19 2 of the samples during illnesses and included HCV OC43 10 0 and HCV NL63 9 6 In addition HCV was commonly detected together with other respiratory viruses 6 5 Of all HCVillnesses n542 33 3 in cluded wheezing however HCV NL63 had a significantly higher wheez ing rate compared to HCV OC43 37 5 vs 4 5 p 5 0 028 Children who had at least one HCV NL63 illness during infancy were significantly morelikelyto haveasthma at age6 comparedtothosewithoutHCV NL63 illnesses 52 2 vs 25 0 p 5 0 007 Having either HCV OC43 or HMPV was not a risk factor for asthma at age 6 CONCLUSIONS Improvements in viral diagnostics has allowed for identification of viruses that are difficult to culture In infancy HCV NL63 HCV OC43 and HMPV were significant causes of wheezing ill nesses however onlyHCV NL63wassignificantlyassociatedwithasthma development at age six Funding NIH grants M01 RR03186 R01 HL61879 and P01 HL70831 575 Seasonal Distribution Of Respiratory Viruses In Pediatric Asthma In Trinidad West Indies J Matthew 1 L M PintoPereira 1 T E Pappas 2 C Swenson 2 K Grindle 2 K Roberg 2 R F Lemaske Jr 2 W Lee 2 J E Gern 2 1 The University of the West Indies St Augustine TRINIDADAND TOBAGO 2 University of Wisconsin Madison Madison WI RATIONALE The seasonal distribution of respiratory viruses associated with pediatric asthma is well documented in temperate climates but has not received similar attention in the tropics METHODS Nasal specimens were collected during the dry n 5 38 JanuarytoMay andrainy n5112 JunetoDecember seasonsfromasth matic children 2 16 years who a presented to A PIV1 Rainy n51 0 9 and rhinovirus Dry n56 15 8 Rainy n5 19 17 0 weredetectedthroughouttheyear Allspecimenswerenegative forRSVA influenzaB coronavirus229E 1 University ofWisconsinSchoolofMed icine and Public Health Madison WI 2 University of Wisconsin Hospital Madison WI 3 University of Wisconsin Hospital Madison WI 4 Univer sity of Wisconsin Madison WI RATIONALE We hypothesize that children identified as wheezers will present with increased levels of inflammatory cytokines and decreased levels of anti viral cytokines in nasal lavage samples during symptomatic RVinfectioncomparedtonon wheezersduringsymptomaticRVinfection METHODS To test this hypothesis children enrolled in the Childhood Origins of ASThma COAST project provided nasal lavage samples dur ing symptomatic respiratory infections and at scheduled well visits at ages 5 and 6 years Children with a history of wheezing were sampled when well during an uncomplicated cold and during a wheezing illness Children with no history of wheezing were sampled when well and ruing an uncomplicated cold Innate cytokine profiles were assayed using a BeadlyteC210HumanMulti CytokineFlexKit anddetectionofthecytokines wereevaluatedusingtheLuminex100C228ISforthefollowingcytokines IL 6 IL 10 IL 12 p40 IFN g IFN a andTNF a Interleukin 8levelswere evaluated by ELISA RESULTS Interleukin 6 IL 10 IFN g and IL 8 were presentin measur able concentrations Concentrations of IL 6 were significantly higher dur ing symptomatic RV infection than when well 14 6 vs 9 91 p 0 001 The detectability of IL 10 and IFN g were significantly higher during symptomatic RV infection than when well 23 9 vs 5 0 p50 045 54 3 vs 0 p50 022 respectively Concentrations of IFN g tended to be higher in non wheezers than in wheezers during symptomatic RVinfection 13 9 vs 8 1 p50 077 CONCLUSIONS During symptomatic RV illness non wheezers pre sented with higher levels of IFN g than wheezers suggesting the possibil ity that wheezersmay have a depressed anti viral response which may not be as effective at fighting viral infections Funding NIH 577 Myosin Light Chain Kinase MYLK Variants that Confer Increased Risk of Sepsis and Acute Lung Injury are Associated with Asthma and Associated Phenotypes L Gao 1 A Grant 1 P Chi 1 P Gao 1 M Stockton 1 H Watson 2 N N Hansel 3 G Diette 3 G Dunston 4 R A Mathias 5 A Togias 1 R Brower 3 J Sevransky 3 J P Maloney 6 M Moss 6 C Shanholtz 7 J G N Garcia 8 T H Beaty 9 K C Barnes 1 1 Johns Hopkins Asthma and Allergy Ctr Baltimore MD 2 University of the West Indies West Indies BARBA DOS 3 Division of Pulmonary and Critical Care Medicine The Johns Hopkins University Baltimore MD 4 National Human Genome Center at Howard University Washington DC DC 5 National Human Genome Research Institute Baltimore MD 6 Division of Pulmonary and Critical Medicine University of Colorado Health Sciences Center Denver CO 7 University of Maryland School of Medicine Baltimore MD 8 Depart ment of Medicine The University of Chicago Biological Sciences Chi cago IL 9 Department of Epidemiology Johns Hopkins University Bloomberg School of Public Health Baltimore MD RATIONALE Asthma is a heritable trait characterized by lower airway smooth muscle contraction and inflammation Myosin light chain kinase isamultifunctionalproteininvolvedinregulationofbronchialcontractility andotheractivitiesrelevanttoasthma PreviouslyweidentifiedMYLKvar iants haplotypes that confer risk for sepsis and acute lung injury ALI J ALLERGY CLIN IMMUNOL JANUARY 2007 S146 Abstracts SUNDAY