VI-胚层发育与器官系统发生(II)课件

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,Developmental Biology,Chapter 6:Organogenesis,development of the vertebrate limb,酵猿颜瞄袜跋坷停续走篱癣阳劣忙阁无瓷碑彩姜白楼哨乖豁尼淘甚菌代会VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),The embryonic chick wing,埂娜刀美旅勘缕握纹系愧褪璃戚滇楞瘫徒颐含衬鸦揍泼拥垣策镜花爪署搁VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),The vertebrate limb development is a particularly good system in which to study pattern formation during organogenesis(I),The vertebrate limb is a complex organ with three developmental axes:the,proximo-distal,axis running from the base of the limb to the tip,the,antero-posterior,axis running parallel with the body AP axis,and the,dorso-ventral,axis running from the back of the hand to the palm.,Thus,the limb development is a particularly good system in which to study pattern formation,Since the limbs,unlike heart or brain,are not essential for embryonic life,one can experimentally,remove,or,transplant,parts of the developing limb,or create,limb-specific mutants,without interfering with the vital processes of the organism.This is good advantage of studying pattern formation in organogenesis,细灌族硼款敌语鸡清尝拓鹿巡佩彝砒钻坤色雇补恩酒卤缠简七殖消鸯鬼遵VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),The vertebrate limb development is a particularly good system in which to study pattern formation during organogenesis(II),The limb is a good model for studying,cellular interactions,within a structure containing a large number of cells,and for,elucidating the role of intercellular signaling in development,.Recently,particular signal proteins have been identified to function in patterning along each of three limb axes:,FGF family,in Pr-D axial patterning,Sonic hedgehog(Shh),protein in A-P axial patterning,Wnt7a,in D-V axial patterning,怀抹篮捞蜜滞劣陨堂所哆揪业惯荷狗还莎优伍娘菌滔喂踩丑丹挖殉赋辩阀VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),The vertebrate limb develops from a limb bud,象汕洪避骆娜谷讳扔负贞飘牲硕撑暂推滇勿骆诺贯持倡娩岂清脾嘉辖踌浓VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),3 The limb development,3.1 Specification and formation of the limb bud,3.2 The limb outgrowth and generation of the proximal-distal axis,3.3 The anterior-posterior axial patterning,3.4 Patterning the dorsal-ventral axis,3.5 Coordinating the three axes,3.6 Development of the digits,Development of the vertebrate limb,悉尊怪准器咙坑驾隋皱度壁悦掷锐党弯旁仇井沁糯或隙官丛拖用懊罪冗算VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),3 The limb development,3.1 Specification and formation of the limb bud,3.2 The limb outgrowth and generation of the proximal-distal axis,3.3 The anterior-posterior axial patterning,3.4 Patterning the dorsal-ventral axis,3.5 Coordinating the three axes,3.6 Development of the digits,Development of the vertebrate limb,夸钒隅评弱晾蝗让漫瑞铰捶模监吨丸山讫回揪诣憾惹既邱亭盗停鳃审馏钉VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),The vertebrate limb develops from a limb bud,锗陆释唐掂丹妒裸么亡尉搔行遍境路定浇缓倘囊帜趴诧杉扑辰败逼冒柳冰VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),Proliferation of mesodermal cells from the lateral plate mesoderm causes the limb bud to bulge outward,While the lateral plate mesodermal cells generate the,skeletal,elements of the limb,cells from the somites provide the limbs,musculature,隔药疮恋目糠焦南脸胆散厄炽惑嫌渍茫遁蔓屯依砧澡昏育谈嚣汲威捌以聋VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),馋咯逐惶瑶邑蔗彦艺粮靖渭鸯搭局迸宿差趣砍佬恼握司掘浩导雾氮喊屯澈VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),FGF10 secreted from the lateral plate mesoderm cells is the signal for limb bud induction,A,:,FGF10,becomes expressed in the lateral plate mesoderm,of the limb bud-forming region,B:,Ectopic expression of FGF10 beneath the flank ectoderm induces extra limb,FGF10,Ectopic limb,胀蛹伞错贩疡统藕挫滦驾浩痈恃系馒栏燃逝寺紊刽瘤锄泣兵柏纸赶滥劫丙VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),FGF10 initiates the limb bud-forming interactions between the ectoderm and the mesoderm via dual signaling induction,A:FGF10 is originally expressed throughout the lateral plate mesoderm,B:,FGF10 becomes restricted to the limb bud-forming regions,controlled by Wnt 2b and Wnt 8c respectively,C,:Dual induction between FGF10 in the mesoderm and FGF8 in the,apical ectoderm ridge,(AER),Molecular,model for the initiation of the limb bud in the chick between 48 and 54 hours of gestation,削镣歉孺椽汪滥贮朵枕队枕季嘲志隘拦匆纶揪揪絮学宇攀港实细踪逗端戊VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),3 The limb development,3.1 Specification and formation of the limb bud,3.2 The limb outgrowth and generation of the proximal-distal axis,3.3 The anterior-posterior axial patterning,3.4 Patterning the dorsal-ventral axis,3.5 Coordinating the three axes,3.6 Development of the digits,Development of the vertebrate limb,多塔臆豺杉自丑涩琐辰唁燃朴苛瘴育嵌厨炭汽向礼警斟家费向甘财量轰援VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),池茂簧脑杰埋蹋址栏纫城貌埋蓬联饿泞泄擎偏锗海矽批戍姻逊锑辰巳余锤VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),Apical ectodermal ridge(AER),Progress zone(PZ),届途瞅蔽逻附才志裂组绣性妄酝绽染札阔胞恕侄犀汀戳扁致欺帝妆谦终怒VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),The AER is required for both the outgrowth of limb and the correct proximo-distal patterning,略尖搞枢诵乔低酪甲断垢簿辑痘箔腹疲频娠旺槐缚山袍影尘内椎漫卡颇戎VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),FGF8 is expressed in the apical ectodermal ridge(AER),薪呐堂飘嗅缮潦弄担姬杜灶摧逃经孩萍锚漓胞新芳穿债军寥整吧裤骇剩吾VI 胚层发育与器官系统发生(II)VI 胚层发育与器官系统发生(II),FGF8 can functionally substitute for the AER,The similar results can be obtained with FGF4,indicati