(参考ppt课件)自噬检测方法

自噬检测方法1 自噬检测方法1 2233常用细胞器标记物4常用细胞器标记物45566自噬体检测：1.透射电镜2.免疫荧光观察LC3斑点,（P62辅助说明）；3.WB检测LC3+P62,并增加自噬降解抑制剂处理组。实验顺序：3 17自噬体检测：7自噬流评价：1.双标LC3,红色斑点/黄色斑点;2.LC3与lamp1的共定位；3.DQ-BSA;4.稳转GFP-LC3细胞中检测游离GFP8自噬流评价：8Fusion of autophagosomes with endosomes/lysosomes is mediated by the class C VPS complex,Rab GTPase and the SNARE complex.Cai,H.,Reinisch,K.&Ferro-Novick,S.Coats,tethers,Rabs,and SNAREs work together to mediate the intracellular destination of a transport vesicle.Dev.Cell 12,671682(2007).Itakura,E.,Kishi-Itakura,C.&Mizushima,N.The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.Cell 151,12561269(2012).Takts,S.et al.Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in Drosophila.J.Cell Biol.201,531539(2013)机制研究：CO-IP比较各组处理后stx17结合的SNAP29和vamp89Fusion of autophagosomes with