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AFU与AFP联合检测在诊断原发性肝癌中的应用 目的 探讨血清-L-岩藻糖苷酶(AFU)及甲胎蛋白(AFP)对原发性肝癌(PHC)的诊断价值。方法 分别测定29例PHC患者、28例肝硬化患者、103例其他肝损伤疾病患者的AFU及AFP含量。 结果 AFU与AFP在PHC中的诊断率分别为86.2%和72.3%,在AFP阴性的PHC患者中,AFU的阳性率为84%,两者联检的总阳性率为93.1%。结论 AFU与AFP联合检测对提高PHC的诊断率有重要价值。原发性肝癌;-L-岩藻糖苷酶;甲胎蛋白ObjectiveTo study the significance of serum-L-fucosidase (AFU) andfetoprotein(AFP) in the diagnosis of primary hepatic carcinoma(PHC). MethodsThe activities of serum AFU and AFP were measured in 29 cases ofPHC ,28 cases of liver cirrhosis ,103 cases of others liver damage. ResultsThe activity in patients with PHC were significantly higher than any other groups. The diagnostic sensitivities of serm AFU and AFP in patients with PHC were 86.2% and 72.3% respectively. Get in the AFP negative gender inside,AFU is 84.0%,and the combined diagnostic sensitivity of AFU and AFP reached the level of 93.1%.ConclusionThe serum AFU and AFP were a useful marker for the diagnosis of PHC.-L-fucosidase;alpha fetoprotein;primary hepatic carcinomaAFP是诊断原发性肝癌(PHC)最特异的肿瘤标志物,但并非所有肝癌患者都能分泌大量AFP,根据报道早期PHC的40%及晚期15%20%患者血清AFP水平是正常的1。AFU是存在于多种组织中的一种溶酶体酶,近年来AFU在PHC患者血清中高水平检出而受到广泛关注2,3,现就血清AFU与AFP对PHC诊断意义进行探讨。1资料与方法1.1研究对象PHC 29例,肝硬化28例,以上患者均为本院住院病人,其诊断经病理及影像学证实,肝损伤(非PHC、肝硬化)患者103例。1.2方法与试剂病人清晨空腹抽取静脉血3ml分离血清,在-20保存待测。血清AFU单位以u/ml表示,以AFU10作为判定PHC的临界标准,采用生化比色法测定,试剂、质控由G.CELL公司提供,AFP单位以ng/ml表示,以AFP20作为临界标准,采用EMI法测定,试剂、质控、标准液由雅培公司提供。1.3仪器CL7200全自动生化分析仪,雅培IMXsystem发光分析仪。
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