电针对坐骨神经损伤大鼠脑源性神经营养因子的影响3800字

电针对坐骨神经损伤大鼠脑源性神经营养因子的影响3800字 摘要：目的 观察电针对坐骨神经损伤（SNI）大鼠脑源性神经营养因子（BDNF）的影响，探讨其治疗SNI的生物学机制。方法 选取成年雄性Wistar大鼠50只，分离并切断大鼠坐骨神经后于体式显微镜下将两侧断端拉入神经再生室内造模。大鼠随机分为正常组、假手术组、模型组和电针组。电针组进行电针治疗，连续28 d。治疗结束后，HE染色观察神经再生，免疫荧光检测神经组织和脊髓BDNF的表达，ELISA检测血清BDNF的表达。结果 电针组轴突再生数量明显多于模型组，且大体轮廓较清晰；与模型组比较，电针组可促进大鼠神经组织、脊髓和血清BDNF的表达（P0.01）。结论 电针可能通过上调神经损伤后BDNF的表达，促进大鼠SNI的再生修复。 毕业关键词：电针；坐骨神经损伤；脑源性神经营养因子；轴突；大鼠DOI：10.3969/j.issn.1005-5304.2017.06.015中图分类号：R245 文献标识码：A 文章编号：1005-5304（2017）06-0060-04Abstract： Objective To explore the effects of electroacupuncture on brain derived neurotrophic factor （BDNF） of rats with sciatic nerve injury （SNI）； To discuss its biological mechanism for treatment of SNI. Methods Fifty adult male Wistar rats were chosen， and the sciatic nerves of rats were cut off and pulled on both sides of the cut ends into nerve regeneration chamber. The rats were randomly divided into normal group， sham-operation group， model group， and electroacupuncture group. In the electroacupuncture group， the rats were treated by electroacupuncture for 28 days. After the treatment， the nerve regeneration was observed through HE staining. Immunofluorescence was used to analyze the expression changes of BDNF in the nerve tissue and spinal cord. ELISA was used to observe the changes of expression of serum BDNF. Results The amount of axon regeneration in the electroacupuncture group was obviously more than that in the model group， and the outline of the tissue more clear. Electroacupuncture could promote the expression of BDNF in the nerve， spinal cord and serum of SNI of rats compared with model group （P0.01）. Conclusion Electroacupuncture can promote the repairment and regeneration of SNI in rats by upregulating the expression of BDNF.Key words： electroacupuncture； sciatic nerve； brain derived neurotrophic factor； axon； rats周?神经损伤（PNI）属中医学“伤筋”“痿证”范畴。诸病源候论?金疮伤筋断骨候云：“夫金疮始伤之时，半伤其筋，荣卫不通，其疮虽愈合后，仍令痹不仁也。”目前治疗方法有神经吻合技术、细胞移植及相关药物应用等，但神经功能的恢复仍不理想1-2。实验和临床研究表明，电针在损伤神经的功能恢复方面疗效肯定和优势明显，能明显提高PNI患肢的肌力，促进神经功能的恢复，减轻后遗症3-4。基金项目：国家自然科学基金（81373754）通讯