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SM0671PageRuler Prestained Protein LadderA prestained SDS-PAGE MW marker with contrasting colored reference bands at 10 and 70kDa.ISO-1Q0-r阳-55-.35-15-25一二二二二二-10 -The Thermo Scie ntific PageRuler Prestai ned Prote is a mixture of ten (10) blue-, ora nge- and gree n-s recomb inant prote ins (10 to 170kDa) for use as siz sta ndards in protein electrophoresis (SDS-PAGE) Wester n blott ing.BlotThis presta ined protein MW marker is desig ned fo mon itori ng the progress of SDS-polyacrylamide ge electrophoresis, for assess ing tra nsfer efficie ncy o nylon and nitrocellulose membranes, and for estim approximate size of separated prote ins that have b visible with gel stains or Western blot detection reagents. The ladder contains one orange refer at 70kDa and one gree n band at 10kDa.Highlights: Size range 一 10 protei ns spa nning 10 to 170kDa Ready-t o-use 一 supplied in a load ing buffer for direct load ing on gels; no n eed to boil Sharp bands 一 color-coded bands of similar inten sity for easy visualizatio n Quality tested -each lot evaluated by SDS-PAGE and Western blotting Two reference bands 一 ora nge at 70kDa and gree n at 10kDa Membrane-compatible colored bands tra nsfer to membra nes for Wester n blott ingIncludes:Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25C), 1mM EDTA, 2% SDS, 11mM NaN3 and 33% glycerol.Applications:Mon itor ing protein migratio n duri ng SDS-polyacrylamide gel electrophoresis Mon itori ng protein tra nsfer on to membra nes after Western blott ingSizing of proteins on SDS-polyacrylamide gels and Western blotsProduct Details:i?0- 13Q-100-70 *-55 -40 一35 -45 -15 -*10 kDaBlotb Sm1841SDS-PAGE band profile of the Thermo Scientific PageRuler Prestained Protein Ladder. Images are from a 4-20% Tris-glycine gel (SDS-PAGE) and subseque nt tra nsfer to membra ne.Spectra Multicolor Broad Range Protein LadderA prestained, 4-color, SDS-PAGE MW marker especially for proteins between 10 and 260kDa.The Thermo Scie ntific Spectra Multicolor Broad Range Prote in Ladder is a 4-color prote in sta ndard containing 10 presta ined recomb inant prokaryotic prote ins (10 to 260kDa) for use as size sta ndards in gel electrophoresis and Western blott ing.This presta ined protein MW marker is desig ned for mon itori ng the progress of SDS-polyacrylamide gel electrophoresis, for assess ing tra nsfer efficie ncy on to PVDF, nylon and n itrocellulose membra nes, and for estimat ing the approximate size of separated prote ins that have bee n made visible with gel stains or Western blot detection reagents. Four different chromophores (blue, orange, gree n, pink) are bound to the differe nt comp onent prote ins, produc ing a brightly colored ladder with an easy-to-remember patter n.Highlights: Size range - 10 protei ns spa nning 10 to 260kDa Multicolor 一 four differe nt colors for un ambiguous ban d-size assig nment Ready-t o-use 一 supplied in a load ing buffer for direct load ing on gels; no n eed to boil Sharp bands 一 color-coded bands of similar inten sity for easy visualizatio n Quality tested -each lot evaluated by SDS-PAGE and Western blotting Membrane-compatible 一 colored bands tra nsfer to membra nes for Wester n blott ingIncludes: Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.Applications: Mon itor ing protein migratio n dur ing SDS-polyacrylamide gel electrophoresis Mon itori ng protein tra nsfer on to membra nes after Western blott ing Siz ing of prote ins on SDS-polyacrylamide gels and Wester n blots-260 -二-140-70 50 40 35 -25 _ 15 -10 -kDaBlotSDS-PAGE band profile of the Thermo ScientificSpectra Multicolor Broad Range Protein Ladder.Sm1811Images are from a 4-20% Tris-glyc ine gel (SDS-PAGE) and subseque nt tra nsfer to membra ne.PageRuler Plus Prestained Protein LadderA prestained SDS-PAGE MW marker with contrasting colored reference bands at 10,25 and 70kDa.The Thermo Scie ntific PageRuler Plus Presta ined Protein-250Ladder is a mixture of nine (9) blue-, ora nge- andgree n-sta ined recomb inant protei ns (10 to 250kDa) for use-IDOEas size sta ndards in protein electrophoresis (SDS-PAGE)and Wester n blott ing.This presta ined protein MW marker is desig ned forJmon itori ng the progress of SDS-polyacrylamide gelelectrophoresis, for assess ing tra nsfer efficie ncy on to PVDF,Se-lJO4V*Blotnylon and n itrocellulose membra nes, and for estimat ing theapproximate size of separated prote ins that have bee n madevisible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except prote ins of two refere nee bands of 70kDa and 25kDa that are colored with an ora nge dye and one gree n refere nee band of 10kDa. PageRuler Plus Presta ined Prote in Ladder is ready to use: no heati ng, further diluti on or additi on of a reduc ing age nt is required before load ing onto a gel.Highlights: Size range 一 nine prote ins spa nning 10 to 250kDa Ready-t o-use 一 supplied in a load ing buffer for direct load ing on gels; no n eed to boil Sharp bands 一 color-coded bands of similar in tesity for easy visualizati on Quality tested -each lot evaluated by SDS-PAGE and Western blotting Bright reference bands 一 ora nge at 70 and 25kDa, and gree n at 10kDa Membrane-compatible colored bands tra nsfer to membra nes for Wester n blott ingIncludes: Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.Applications: Mon itor ing protein migratio n dur ing SDS-polyacrylamide gel electrophoresis Mon itori ng protein tra nsfer on to membra nes after Western blott ing Siz ing of prote ins on SDS-polyacrylamide gels and Wester n blotsGelkDa-250“30-100-55-151QBlotSDS-PAGE band profile of the Thermo Scientific PageRuler Plus Prestained Protein Ladder.Images are from a 4-20% Tris-glyc ine gel (SDS-PAGE) and subseque nt tra nsfer to membra ne.
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